Three-Dimensional Ingrowth of Bone Cells Within Biodegradable Cryogel Scaffolds in Bioreactors at Different Regimes
Three-Dimensional Ingrowth of Bone Cells Within Biodegradable Cryogel Scaffolds in Bioreactors at Different Regimes
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DOI：
10.1089/ten.tea.2007.0277
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发表时间：
2008-10-01
影响因子：
4.1
通讯作者：
Piskin, Erhan
中科院分区：
文献类型：
--
作者：
Bolgen, Nimet;Yang, Ying;Piskin, Erhan
在生物反应器中，在不同的制度（静态，灌注，和压缩灌注）的三维细胞向内生长的生物可降解的冻凝胶支架交联的甲基丙烯酸2-羟乙酯（HEMA）-乳酸-葡聚糖与互连的大孔进行了研究。在这些研究中使用成骨细胞样细胞系（MG 63）。通过显微镜技术（光、SEM、TEM和激光扫描共聚焦）检查在选定时间后从生物反应器中采集的样品。发现细胞培养条件不仅对细胞形态（如细胞附着和向内生长的程度）而且对细胞活性具有显著影响。动态条件（灌注和/或压缩）大大改善了细胞向内生长和细胞外基质（ECM）的合成。碱性磷酸酶活性结果证实了动态条件对骨细胞的积极影响。
Three-dimensional cell ingrowth within biodegradable cryogel scaffolds made of cross-linked 2-hydroxyethyl methacrylate (HEMA)-lactate-dextran with interconnected macropores was studied in bioreactors at different regimes (static, perfusion, and compression-perfusion). An osteoblast-like cell line (MG63) was used in these studies. The samples taken after selected times from the bioreactors were examined by microscopy techniques (light, SEM, TEM, and laser scanning confocal). The cell culture conditions were found to have a significant impact not only on the cell morphology, such as the extent of cell attachment and ingrowth, but also on cellular activities. Dynamic conditions (perfusion and/or compression) greatly improved cell ingrowth and extracellular matrix (ECM) synthesis. Alkaline phosphatase activity results confirmed the positive effect of dynamic conditions on bone cells.