ras‐family gene mutations in neoplasia of the ampulla of vater
ras‐family gene mutations in neoplasia of the ampulla of vater
复制标题
肝壶腹肿瘤中ras家族基因突变
DOI：
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发表时间：
1994
影响因子：
6.4
通讯作者：
R. Accolla
中科院分区：
文献类型：
--
作者：
A. Scarpa;G. Zamboni;A. Achille;P. Capelli;G. Bogina;C. Iacono;G. Serio;R. Accolla
采用单链构象多态性分析和聚合酶链反应扩增DNA片段直接测序的方法，对17例乏特壶腹上皮性肿瘤中Ha-、Ki-和N-ras癌基因第一和第二外显子的突变进行了研究。本组包括12例垂体型腺癌，3例绒毛状腺瘤，1例乳头状癌和1例神经内分泌癌。6例病例（35%）包含ras突变，影响2例腺瘤和3例癌中Ki‐ras的密码子12，以及I例腺瘤中N‐ras的密码子12。所有的突变都发现于腺瘤和有腺瘤区域的癌症中，而没有腺瘤区域的病例中没有一个包含突变。这表明，壶腹癌代表异质性疾病的存在或不存在的腺瘤区，其中与腺瘤区，相对于激活ras基因的存在。Ki-ras突变病例包括4例肿瘤中的3例，主要涉及十二指肠内胆管，因此表明一部分Ki-ras突变的壶腹癌可能对应于起源于壶腹胆管成分上皮的癌。
Mutations in the first and second exons of Ha‐, Ki‐ and N‐ras oncogenes were investigated in 17 epithelial tumors of the ampulla of Vater by single‐strand conformation polymorphism analysis and direct sequencing of DNA fragments amplified by polymerase chain reaction. The panel included 12 intestinaltype adenocarcinomas, 3 villous adenomas, I papillary carcinoma and I neuroendocrine carcinoma. Six cases (35%) contained ras mutations, affecting codon 12 of Ki‐ras in 2 adenomas and 3 carcinomas, and of N‐ras in I adenoma. All mutations were found in adenomas and among cancers with adenomatous areas, whereas none of the cases lacking adenomatous areas contained mutations. This suggested that ampullary cancers represent heterogeneous diseases with respect to the presence or absence of adenomatous areas and, among those with adenomatous areas, with respect to the presence of activated ras genes. Ki‐ras mutated cases included 3 of 4 tumors which mainly involved the intraduodenal bile duct, thus suggesting that a proportion of Ki‐ras ‐mutated ampullary cancers might correspond to those originating from the epithelium of the bile duct component of the ampulla.
影响因子：
16.6
作者：
M. Barbacid
通讯作者：
M. Barbacid
DOI：
10.1073/pnas.85.23.9268
发表时间：
1988-12-01
影响因子：
11.1
作者：
NERI, A;KNOWLES, DM;DALLAFAVERA, R
通讯作者：
DALLAFAVERA, R