Prolonged cyclic strain inhibits human endothelial cell growth.
Prolonged cyclic strain inhibits human endothelial cell growth.
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DOI：
10.2741/e761
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Durante W
中科院分区：
文献类型：
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作者：
Peyton KJ;Liu XM;Durante W
由于脉动血流导致的血管直径的周期性变化，血管内皮持续暴露于周期性机械应变。由于新出现的证据表明，循环应变在调节内皮细胞功能中起着不可或缺的作用，本研究确定了应用生理方案的循环应变（6%，1赫兹）是否会影响人动脉内皮细胞的增殖。人真皮微血管或人主动脉内皮细胞长时间暴露于周期性应变长达7天，导致细胞生长显着下降。应变介导的抗增殖作用与内皮细胞在细胞周期的G2/M期的停滞有关，不涉及细胞脱离或细胞毒性，并且是由于p21的诱导。有趣的是，内皮细胞生长的抑制与应变方案无关，因为持续或间歇性施加6%应变也能够阻断内皮细胞增殖。慢性生理周期性应变抑制内皮细胞生长的能力代表了一种以前未被认识的机制，通过这种机制，血液动力学力将这些细胞维持在静止、非增殖状态。
The vascular endothelium is continuously exposed to cyclic mechanical strain due to the periodic change in vessel diameter as a result of pulsatile blood flow. Since emerging evidence indicates the cyclic strain plays an integral role in regulating endothelial cell function, the present study determined whether application of a physiologic regimen of cyclic strain (6% at 1 hertz) influences the proliferation of human arterial endothelial cells. Prolonged exposure of human dermal microvascular or human aortic endothelial cells to cyclic strain for up to 7 days resulted in a marked decrease in cell growth. The strain-mediated anti-proliferative effect was associated with the arrest of endothelial cells in the G2/M phase of the cell cycle, did not involve cell detachment or cytotoxicity, and was due to the induction of p21. Interestingly, the inhibition in endothelial cell growth was independent of the strain regimen since prolonged application of constant or intermittent 6% strain was also able to block endothelial cell proliferation. The ability of chronic physiologic cyclic strain to inhibit endothelial cell growth represents a previously unrecognized mechanism by which hemodynamic forces maintain these cells in a quiescent, non-proliferative state.