Steroid metabolome in plasma from the umbilical artery, umbilical vein, maternal cubital vein and in amniotic fluid in normal and preterm labor
Steroid metabolome in plasma from the umbilical artery, umbilical vein, maternal cubital vein and in amniotic fluid in normal and preterm labor
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DOI：
10.1016/j.jsbmb.2009.10.012
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发表时间：
2010-08-01
影响因子：
4.1
通讯作者：
Starka, Luboslav
中科院分区：
文献类型：
--
作者：
Hill, Martin;Parizek, Antonin;Starka, Luboslav
The boost in placental production of CRH in late pregnancy is specific for human. CRH receptors are expressed in the fetal zone of the fetal adrenal (FZFA). Hence, we evaluated the associations between the steroid metabolome and gestational age (GA). The levels of 69 steroids and steroid polar conjugates such as 3 beta-hydroxy-5-ene steroids (3 beta OH5S), 3-oxo-4-ene steroids (3O4S), progesterone 5 alpha/beta-reduced metabolites, 20 alpha-hydroxy-metabolites of C21 steroids, C19 5 alpha/beta-reduced metabolites, 7 alpha/beta-hydroxy-metabolites of 3 beta OH5S, estrogens and 16 alpha-hydroxy-metabolites of 3 beta OH5S and 3O4S, were measured by GC-MS in plasma from the umbilical artery (UA), umbilical vein (UV), and maternal cubital vein (MV) and in amniotic fluid (AF) in 12 women at normal labor and 38 women at preterm labor due to pathologies unrelated to steroid status. Using multivariate regression, prediction models for GA were completed for the individual body fluids. The conjugated 3 beta OH5S (the key products of the FZFA), estrogens, some polar conjugates of progesterone 5 alpha/beta-reduced metabolites and some steroid 7 alpha/beta- and 16 alpha-hydroxy-metabolites showed strong positive correlations with the GA. The predictivity decreased in the following sequence UV (R = 0.950), UA (R = 0.945), MV (R = 0.895), and AF (R = 0.891). Although the predictivity of steroids in maternal blood was slightly less effective when compared with the UV and UA, it was the best solution for further practice. (C) 2009 Elsevier Ltd. All rights reserved.
