Serum SP-D is a marker of lung injury in rats
Serum SP-D is a marker of lung injury in rats
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DOI：
10.1152/ajplung.00421.2000
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发表时间：
2002-04-01
影响因子：
4.9
通讯作者：
Mason, RJ
中科院分区：
文献类型：
--
作者：
Pan, TL;Nielsen, LD;Mason, RJ
肺表面活性物质蛋白D（SP-D）在肺泡II型和细支气管上皮细胞中表达，并分泌到肺泡和传导气道中。然而，SP-D也在血清中被测量，并且在急性呼吸窘迫综合征、肺纤维化和肺泡蛋白沉积症患者中增加。为了证明可以在大鼠血清中测量SP-D，我们向大鼠滴注角质形成细胞生长因子，其产生II型细胞增生和支气管肺泡灌洗液（BALF）中SP-D的增加。为了评估血清SP-D作为肺损伤的生物标志物，我们检查了几种损伤模型。在用1单位博莱霉素处理的大鼠中，血清SP-D在滴注后第3、7、14和28天升高，并且通过原位杂交检测到局灶性区域SP-D mRNA增加。然而，当表达标准化为全肺18 S rRNA时，全肺SP-D mRNA没有增加。2个单位博莱霉素滴入后，血清SP-D水平升高，BALF和肺组织匀浆中SP-D也升高。在另一种亚急性损伤模型中，百草枯加氧处理的大鼠血清SP-D升高。最后，为了评估急性肺损伤，我们向大鼠滴注HCl;在滴注后4小时，SP-D增加。我们的数据表明，血清SP-D可能是一个有用的指标肺损伤和II型细胞增生的大鼠。
Pulmonary surfactant protein D (SP-D) is expressed in alveolar type II and bronchiolar epithelial cells and is secreted into alveoli and conducting airways. However, SP-D has also been measured in serum and is increased in patients with acute respiratory distress syndrome, pulmonary fibrosis, and alveolar proteinosis. To demonstrate that SP-D can be measured in rat serum, we instilled rats with keratinocyte growth factor, which produces type II cell hyperplasia and an increase in SP-D in bronchoalveolar lavage fluid (BALF). To evaluate serum SP-D as a biomarker of lung injury, we examined several injury models. In rats treated with 1 unit of bleomycin, serum SP-D was elevated on days 3, 7, 14, and 28 after instillation, and SP-D mRNA was increased in focal areas as detected by in situ hybridization. However, there was no increase in whole lung SP-D mRNA when the expression was normalized to whole lung 18S rRNA. After instillation of 2 units of bleomycin, the serum levels of SP-D were higher, and SP-D was also increased in BALF and lung homogenates. In another model of subacute injury, serum SP-D was increased in rats treated with paraquat plus oxygen. Finally to evaluate acute lung injury, we instilled rats with HCl; SP-D was increased at 4 h after instillation. Our data indicate that serum SP-D may be a useful indicator of lung injury and type II cell hyperplasia in rats.