Pretreatment of Corn Stover with the Modified Hydrotropic Method To Enhance Enzymatic Hydrolysis
Pretreatment of Corn Stover with the Modified Hydrotropic Method To Enhance Enzymatic Hydrolysis
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DOI：
10.1021/ef5001634
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发表时间：
2014-07-01
期刊：
影响因子：
5.3
通讯作者：
Fardim, Pedro
中科院分区：
文献类型：
--
作者：
Mou, Hongyan;Li, Bin;Fardim, Pedro
采用二甲苯磺酸钠（SXS）对玉米秸秆进行Hydrotropic预处理，脱除木质素和木聚糖，促进玉米秸秆的酶解糖化。在亲水处理之前处理过乙酸（PAA）[所谓的改性亲水预处理（MHP）]可以使脱木质素效率提高一倍，并显著提高葡聚糖转化率。预处理后，样品经PFI精炼处理进行比较。经mhp处理的玉米秸秆在酶解前补充PFI精制，葡聚糖得率可达87.6%，木聚糖得率为43.7%。利用傅里叶变换红外光谱（FTIR）、x射线衍射仪（XRD）和扫描电镜（SEM）对预处理后的玉米秸秆进行了分析。用FTIR对废液中木质素析出物进行了研究。从FTIR结果可以观察到木质素结构的断裂。XRD分析表明，MHP处理后玉米秸秆的结晶度指数（CrI）有所提高，FTIR分析了预处理样品间纤维素总CrI的降低情况。SEM分析表明，PAA处理通过产生孔隙和使酶与多糖更好地接触来影响玉米秸秆纤维的形态。
Hydrotropic pretreatment using sodium xylene sulfonate (SXS) could remove lignin and xylan from corn stover to enhance enzymatic saccharification. Peracetic acid (PAA) treatment prior to the hydrotropic process [so-called modified hydrotropic pretreatment (MHP)] could double the delignification efficiency and remarkably increase glucan conversion. After pretreatment, samples were treated by PFI refining for comparison. With the supplement of PFI refining before enzymatic hydrolysis of the MHP-treated corn stover, 87.6% of the glucan yield could be achieved and the corresponding xylan yield was 43.7%. In addition, the pretreated corn stover was analyzed by Fourier transform infrared spectroscopy (FTIR), X-ray diffractometer (XRD), and scanning electron microscopy (SEM). The lignin precipitate from the spend liquor was also investigated by FTIR. The cleavage of the lignin structure could be observed from FTIR results. The crystallinity index (CrI) of corn stover after MHP was increased according to XRD analysis, while the reduction of total CrI of cellulose between pretreatment samples was analyzed by FTIR. SEM analysis demonstrated that PAA treatment affected the morphology of corn stover fiber by generating pores and allowing for better contact of the enzyme to polysaccharides.