TYPE-1 ASTROCYTES FAIL TO INHIBIT SCHWANN-CELL REMYELINATION OF CNS AXONS IN THE ABSENCE OF CELLS OF THE O-2A LINEAGE
TYPE-1 ASTROCYTES FAIL TO INHIBIT SCHWANN-CELL REMYELINATION OF CNS AXONS IN THE ABSENCE OF CELLS OF THE O-2A LINEAGE
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DOI：
10.1159/000111651
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发表时间：
1992-03-01
影响因子：
2.9
通讯作者：
BLAKEMORE, WF
中科院分区：
文献类型：
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作者：
FRANKLIN, RJM;CRANG, AJ;BLAKEMORE, WF
雪旺细胞对脱髓鞘中枢神经系统轴突的再生能力受星形胶质细胞的影响。为了进一步研究星形胶质细胞控制中枢神经系统雪旺细胞再髓鞘形成的本质，将含有1型星形胶质细胞和少量雪旺细胞的培养物移植到成年大鼠脊髓无神经胶质细胞损伤处，在此损伤处，宿主对脱髓鞘轴突的反应受到X射线的抑制。将这些O-2A谱系缺失的培养物移植到X射线照射的脱髓鞘损伤处后，雪旺细胞对脱髓鞘轴突进行了广泛的再髓鞘化，这一结果与早期实验中移植体内存在O-2A谱系细胞和/或从宿主组织中招募的结果形成了鲜明对比。这项实验表明，O-2A系细胞的存在是移植的1型星形胶质细胞以一种抑制中枢神经系统轴突广泛的雪旺细胞再髓鞘形成的方式组织所必需的。
The extent to which Schwann cells are able to remyelinate demyelinated CNS axons is influenced by the presence of astrocytes. In order to study further the nature of astrocyte control of Schwann cell remyelination in the CNS, cultures containing type 1 astrocytes and a small proportion of Schwann cells, but depleted of O-2A lineage cells by exposure to cytosine arabinoside and complement-mediated immunocytolysis, were transplanted into glial-free lesions in adult rat spinal cord in which the host response to demyelinated axons was suppressed by X-irradiation. Following transplantation of these O-2A lineage-depleted cultures into X-irradiated, demyelinating lesions, there was extensive remyelination of demyelinated axons by Schwann cells, a result which contrasted with those obtained from earlier experiments in which O-2A lineage cells were present within the transplant, and/or recruited from host tissue. This experiment shows that the presence of O-2A lineage cells is required in order for transplanted type 1 astrocytes to organise in a manner which inhibits extensive Schwann cell remyelination of CNS axons.