A genetic analysis of the cytological region 46C-F containing the Drosophila melanogaster homolog of the jun proto-oncogene
A genetic analysis of the cytological region 46C-F containing the Drosophila melanogaster homolog of the jun proto-oncogene
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DOI：
10.1007/s00438-001-0592-y
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发表时间：
2001-12-01
影响因子：
3.1
通讯作者：
Lim, J
中科院分区：
文献类型：
--
作者：
Goldstein, ES;Treadway, SL;Lim, J
果蝇 2 号染色体右臂上的细胞遗传学区域 46C-F 包含人类 jun 原癌基因的同源物，已进行遗传图谱和表征。 This project led to the identification and characterization of a Jra (jun-related antigen) mutation, which has been described in detail elsewhere.在此期间，使用 EMS、DEB 和伽马射线三种诱变剂分离出 126 个致死品系。对 126 个致死品系的互补分析确定了该地区的 29 个致死互补组，其中 9 个现已与已知基因或表型相关。使用各种小缺失，该区域被细分为十个区间，46C/D 中的七个区间和 46E/F 中的三个区间。十六个 P-clement 系已被映射到该区间，并且与我们的八个互补组等位。其余未识别的互补组已针对关键阶段进行了分析。这是第一个可观察到的缺陷出现和/或死亡发生的时间。有十二个胚胎致死群和七个幼虫致死群。三行显示死亡前肠道和气管发育明显异常。
The cytogenetic region 46C-F on the right arm of Drosophila chromosome 2, which contains the homolog of the human jun proto-oncogene, has been genetically mapped and characterized. This project led to the identification and characterization of a Jra (jun-related antigen) mutation, which has been described in detail elsewhere. Three mutagens, EMS, DEB and gamma-rays, were used to isolate 126 lethal lines for this interval. Complementation analysis of the 126 lethal lines identified 29 lethal complementation groups in the region, nine of which have now been correlated with known genes or phenotypes. The region has been subdivided into ten intervals using various small deletions, seven intervals in 46C/D and three intervals in 46E/F. Sixteen P-clement lines have been mapped to this interval and are allelic to eight of our complementation groups. The remaining unidentified complementation groups have been analyzed for critical phase. which is when the first observable defect arises and/or when death occurs. There are twelve embryonic lethal groups and seven larval lethal groups. Three lines show visible abnormalities in gut and tracheal development prior to death.