Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells.
Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells.
复制标题
连续人骨髓培养：可能的多能干细胞的 Ia 抗原表征。
作者：
M. Moore;H. Broxmeyer;A. Sheridan;P. Meyers;N. Jacobsen;R. Winchester
证实了人CFU-C和BFU-e上存在ia样抗原，并鉴定了一种缺乏粒细胞集落形成能力但在简单悬浮培养中短暂孵育后产生CFU-C的细胞类型。该前cfu -c及其直系后代对具有补体的抗ia血清杀伤极为敏感。相比之下，抗ia血清加补体处理人骨髓，虽然消除了93%-97%的CFU-c和BFU-e，但在连续骨髓培养条件下，并没有阻止这些祖细胞的快速再生和它们的产生。这些研究表明，人类等效的多能干细胞可以在培养中复制数周，并产生固定的祖细胞，如CFU-c和BFU-e。此外，似乎在多能干细胞上不存在ia样抗原，在与各种祖细胞类型的结合中迅速获得，随后在这些祖细胞类型在骨髓中分化时丢失。
The presence of Ia-like antigens on human CFU-C and BFU-e is confirmed and a cell type that lacked immediate capacity for granulocytic colony formation but generated CFU-c after brief incubation in simple suspension culture is identified. This pre-CFU-c, and its immediate progeny, was extremely sensitive to killing by anti-Ia serum with complement. In contrast, anti-Ia serum plus complement treatment of human bone marrow, while eliminating 93%-97% of all CFU-c and BFU-e, did not prevent the rapid regeneration of these progenitor cells and their production for some weeks under the conditions of continuous marrow culture. These studies suggest that the human equivalent of the pluripotential stem cell can replicate for some weeks in culture and generate committed progenitors, such as CFU-c and BFU-e. Furthermore, it would appear that Ia-like antigen is absent on the pluripotential stem cell, is rapidly gained as commitment to the various progenitor cell types occur, and is subsequently lost as these latter undergo differentiation within the marrow.