Therapy modifies cystine kidney stones at the macroscopic scale. Do such alterations exist at the mesoscopic and nanometre scale?
Therapy modifies cystine kidney stones at the macroscopic scale. Do such alterations exist at the mesoscopic and nanometre scale?
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DOI：
10.1107/s1600576714004658
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发表时间：
2014-04-01
影响因子：
6.1
通讯作者：
Matzen, Guy
中科院分区：
文献类型：
--
作者：
Bazin, Dominique;Daudon, Michel;Matzen, Guy
胱氨酸尿症的发病率为1：7000，是遗传性疾病中最常见的结石形成原因，是一个主要的医学问题。随机选择25例胱氨酸尿症患者的胱氨酸结石进行了调查。从晶体学的角度来看，胱氨酸结石由微米大小的微晶组成，这些微晶由纳米晶体的聚集体组成。通过扫描电子显微镜，晶粒的形貌和尺寸进行了描述，而纳米晶体的尺寸进行了研究，通过粉末中子衍射。胱氨酸结石的粉末中子衍射分析和/或扫描电子显微镜检查提供的证据表明，通常碱化碳酸氢钠与高利尿显着降低纳米晶体和微晶的大小，而对于其他治疗，包括碱化药物和硫醇衍生物，数据表明主要是在微晶的拓扑结构的变化。用碳酸氢钠碱化在介观和纳米尺度上影响胱氨酸肾结石，而其他医学治疗仅改变其表面。这种方法可能有助于评估药物和胱氨酸尿患者胱氨酸结石之间的相互作用。
With an incidence of 1:7000 births, cystinuria, the most frequent cause of stone formation among genetic diseases, represents a major medical problem. Twenty-five cystine stones randomly selected from cystinuric patients were investigated. From a crystallographic point of view, cystine stones are composed of micrometre size crystallites, which are made up of an aggregation of nanocrystals. Through scanning electron microscopy, the morphology and size of the crystallites have been described, while the size of the nanocrystals was investigated by means of powder neutron diffraction. Powder neutron diffraction analysis and/or scanning electron microscopy examination of cystine stones provide evidence that usual alkalinization by sodium bicarbonate associated with high diuresis significantly reduces the size of both nanocrystals and crystallites, while for other treatments, including alkalinizing drugs and thiol derivatives, the data suggest mainly changes in the topology of crystallites. Alkalinization with sodium bicarbonate affects cystine kidney stones at the mesoscopic and nanoscopic scales, while other medical treatments only alter their surface. Such an approach may help to assess the interaction between drugs and cystine stones in cystinuric patients.