THE NEUROPEPTIDE CEREBELLIN IS A MARKER FOR 2 SIMILAR NEURONAL CIRCUITS IN RAT-BRAIN
THE NEUROPEPTIDE CEREBELLIN IS A MARKER FOR 2 SIMILAR NEURONAL CIRCUITS IN RAT-BRAIN
复制标题
DOI：
10.1073/pnas.84.23.8692
复制
发表时间：
1987-12-01
影响因子：
11.1
通讯作者：
MORGAN, JI
中科院分区：
文献类型：
--
作者：
MUGNAINI, E;MORGAN, JI
我们在这里报道了神经肽小脑蛋白，一个已知的小脑浦肯野细胞的标记，只有一个重要的小脑外位置，耳蜗核背侧(DCoN)。通过反相高效液相色谱和放射免疫分析，发现大鼠小脑和DCoN中含有类似浓度的该十六肽。免疫细胞化学显示，小脑素样免疫反应仅定位于浦肯野细胞，而在DCoN，小脑素样免疫反应主要见于车轮细胞和锥体神经元的基树突。一些移位的浦肯野细胞也被染色。尽管小脑和DCoN的输入来自不同的大脑区域，但它们的组织结构显示出几个相似之处，其中一个区域存在许多神经细胞类型，而另一个区域则存在假定的同源物。Cerebellim存在于小脑和DCoN的浦肯野和车轮细胞中，没有部位偏爱。这应该与最近的数据一起来看待，这些数据表明，这两类神经元在几个小鼠突变中有着相似的命运(“神经”、“蹒跚”、“浦肯野细胞变性”和“摇摇晃晃”)。这种情况导致我们得出结论，小脑代表了一小部分空间上相关的神经细胞类别的标记，它可能在值得进一步探索的电路功能的一个未知方面发挥作用。
We report here that the neuropeptide cerebellin, a known marker of cerebellar Purkinje cells, has only one substantial extracerebellar location, the dorsal cochlear nucleus (DCoN). By reverse-phase high-performance liquid chromatography and radioimmunoassay, cerebellum and DCoN in rat were found to contain similar concentrations of this hexadecapeptide. Immunocytochemistry with our rabbit antiserum C1, raised against synthetic cerebellim, revealed that cerebellin-like immunoreactivity in the cerebellum is localized exclusively to Purkinje cells, while in the DCoN, it is found primarily in cartwheel cells and in the basal dendrites of pyramidal neurons. Some displaced Purkinje cells were also stained. Although cerebellum and DCoN receive their inputs from different brain regions, their organizations show several similarities, with many neuronal cell types present in one having their presumed homologue in the other. Cerebellim is present in Purkinje and cartwheel cells throughout the cerebellum and the DCoN without site predilection. This should be viewed together with recent data indicating that these two classes of neurons share a similar fate in several murine mutations ("nervous," "lurcher," "Purkinje cell degeneration," and "staggerer"). This situation leads us to conclude that cerebellim represents a marker for a small number of spatially related neuronal cell classes, and that it may play a role in an as yet unknown aspect of a circuit function worthy of further exploration.