The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(L-lactide) on the osteogenesis of mesenchymal stem cells
The synergistic effect of nano-hydroxyapatite and dexamethasone in the fibrous delivery system of gelatin and poly(L-lactide) on the osteogenesis of mesenchymal stem cells
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DOI：
10.1016/j.ijpharm.2016.04.032
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发表时间：
2016-06-30
影响因子：
5.8
通讯作者：
Shabani, Iman
中科院分区：
文献类型：
--
作者：
Amjadian, Sara;Seyedjafari, Ehsan;Shabani, Iman
近年来，电纺纳米纤维支架由于能够模拟天然组织的自然结构，在骨组织工程中得到了广泛的应用。在我们的研究中，纳米纤维的表面特征进行了修改，通过同时电纺丝的合成和天然聚合物使用聚L-乳酸（PLLA）和明胶制造的混合支架（PLLA/明胶）。然后，在第二个实验中，将羟基磷灰石纳米颗粒（nHA）装载在电纺PLLA纳米纤维（PLLA，nHA/明胶）中，并且还将地塞米松（DEX）掺入这些纤维（PLLA，nHA，DEX/明胶）中。通过SEM、FTIR光谱、接触角、拉伸强度、DEX释放曲线和MTT测定对制备的纳米纤维复合支架进行表征。在接种脂肪来源的间充质干细胞后，使用常见的成骨标志物如碱性磷酸酶活性、钙沉积和基因表达来分析制造的支架的骨诱导性和骨传导性。这些结果证实了纳米纤维的所有性能通过改性得到改善。此外，与PLLA/明胶相比，PLLA、nHA/明胶组的干细胞成骨分化增加。从PLLA、nHA、DEX/明胶获得DEX的持续释放，其随后导致更多的成骨分化。PLLA、nHA、DEX/明胶在支持干细胞增殖和成骨分化方面显示出显著的潜力，可作为组织工程和再生医学应用的良好候选材料。(C)2016爱思唯尔B. V.保留所有权利。
Recently, electrospun nanofibrous scaffolds are vastly taken into consideration in the bone tissue engineering due to mimicking the natural structure of native tissue. In our study, surface features of nanofibers were modified through simultaneous electrospining of the synthetic and natural polymers using poly L-lactide (PLLA) and gelatin to fabricate the hybrid scaffold (PLLA/gelatin). Then, hydroxyapatite nanoparticles (nHA) were loaded in electrospun PLLA nanofibers (PLLA,nHA/gelatin) and also dexamethasone (DEX) was incorporated in these fibers (PLLA, nHA, DEX/gelatin) in the second experiment. Fabricated nanofibrous composite scaffolds were characterized via SEM, FTIR spectroscopy, contact angle, tensile strength measurements, DEX release profile and MTT assay. After seeding adipose derived mesenchymal stem cells, osteoinductivity and osteoconductivity of fabricated scaffolds were analyzed using common osteogenic markers such as alkaline phosphatase activity, calcium depositions and gene expression. These results confirmed that all properties of nanofibers were improved by modifications. Moreover, osteogenic differentiation of stem cells increased in PLLA, nHA/gelatin group in comparison with PLLA/gelatin. The sustained release of DEX was obtained from PLLA, nHA, DEX/gelatin which subsequently led to more osteogenic differentiation. Taken together, PLLA, nHA, DEX/gelatin showed significant potential to support the stem cell proliferation and ostogenic differentiation, and can be a good candidates for tissue engineering and regenerative medicine applications. (C) 2016 Elsevier B.V. All rights reserved.