Redox changes during cold acclimation affect freezing tolerance but not the vegetative/reproductive transition of the shoot apex in wheat
Redox changes during cold acclimation affect freezing tolerance but not the vegetative/reproductive transition of the shoot apex in wheat
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DOI：
10.1111/j.1438-8677.2010.00429.x
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发表时间：
2011-09-01
期刊：
影响因子：
3.9
通讯作者：
Galiba, G.
中科院分区：
文献类型：
--
作者：
Soltesz, A.;Timar, I.;Galiba, G.
冷锻炼是冬小麦（Triticum aestivum L.）以达到其基因决定的最大抗冻性，寒冷也满足春化要求。染色体5A是这些性状的主要调节者。本研究的目的是发现是否变化的半细胞氧化还原电位的谷胱甘肽/谷胱甘肽二硫化物（GSH/GSSG）和抗坏血酸/脱氢抗坏血酸（AA/DHA）夫妇的冷驯化诱导的相关的抗冻性和春化要求在一个特定的遗传系统，包括染色体5A置换线。H2 O2和AA的量，和AA/DHA的比例显示了一个快速和短暂的增加，在所有基因型的树冠在驯化的第一周，随后逐渐增加，在随后的2周。GSH的量和它的比例相比，GSSG在第一天迅速下降，而后来这些参数显示出持续缓慢的增加。H2 O2，AA和GSH的浓度，AA/DHA和GSH/GSSG的比例和半细胞还原电位的GSH/GSSG夫妇与抗冻性水平后22天在2 ℃，因此这些参数可能有一个重要的作用，在驯化过程中。与H2 O2和非酶抗氧化剂相比，四种抗氧化酶的脂质过氧化物浓度和活性在第一周内表现出短暂的增加，基因型之间没有显着差异。研究的参数没有显示出任何关系的营养/生殖过渡状态监测顶点形态和春化基因表达。
Cold acclimation is necessary for winter wheat (Triticum aestivum L.) to achieve its genetically determined maximum freezing tolerance, and cold also fulfils the vernalisation requirement. Chromosome 5A is a major regulator of these traits. The aim of the present study was to discover whether changes in the half-cell redox potential of the glutathione/glutathione disulphide (GSH/GSSG) and ascorbate/dehydroascorbate (AA/DHA) couples induced by cold acclimation are related to freezing tolerance and vernalisation requirement in a specific genetic system including chromosome 5A substitution lines. The amounts of H2O2 and AA, and the AA/DHA ratio showed a rapid and transient increase in the crown of all genotypes during the first week of acclimation, followed by a gradual increase during the subsequent 2 weeks. The amount of GSH and its ratio compared to GSSG quickly decreased during the first day, while later these parameters showed a continuous slow increase. The H2O2, AA and GSH concentrations, AA/DHA and GSH/GSSG ratios and the half-cell reduction potential of the GSH/GSSG couple were correlated with the level of freezing tolerance after 22 days at 2 degrees C; hence these parameters may have an important role in the acclimation process. In contrast to H2O2 and the non-enzymatic antioxidants, the lipid peroxide concentration and activity of the four antioxidant enzymes exhibited a transient increase during the first week, with no significant difference between genotypes. None of the parameters studied showed any relationship with the vegetative/generative transition state monitored as apex morphology and vernalisation gene expression.