Immunohistochemical localization of Foxa1 and Foxa2 in mouse embryos and adult tissues
Immunohistochemical localization of Foxa1 and Foxa2 in mouse embryos and adult tissues
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DOI：
10.1016/j.modgep.2004.08.006
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发表时间：
2004-12-01
影响因子：
1.2
通讯作者：
Whitsett, JA
中科院分区：
文献类型：
--
作者：
Besnard, V;Wert, SE;Whitsett, JA
已知叉头盒（Fox）转录因子家族Foxa 1（Hnf-3 α）、Foxa 2（Hnf-3 β）的成员影响内胚层来源的组织（包括肺、肝、胰腺、胃和肠）中的基因表达。在本研究中，我们已经产生了高度特异性的抗体Foxa 1和Foxa 2，并确定其在发展和成年小鼠的表达模式。在来源于前肠和后肠内胚层的组织（肝、肺、胰腺、胃、肠、前列腺和膀胱）的细胞核中检测到Foxa 1和Foxa 2。Foxa 2和Foxa 1也检测到来自外胚层（几个大脑结构和嗅觉上皮）和中胚层起源（肾脏，阴道和子宫，精液和前列腺）在发展过程中的器官。Foxa 1和Foxa 2的共定位和不同的表达位点表明Foxa 1或Foxa 2在形态发生和不同成体器官的功能中具有独特和重叠的作用。(C)2004 Elsevier B. V.保留所有权利。
Members of the Forkhead box (Fox) transcription factors family Foxa1 (Hnf-3alpha), Foxa2 (Hnf-3beta) are known to influence gene expression in endodermally derived tissues including lung, liver, pancreas, stomach, and intestine. In the present study, we have generated highly specific antibodies for Foxa1 and Foxa2 and determined their expression patterns in the developing and adult mouse. Foxa1 and Foxa2 were detected in the nuclei of tissues derived from both foregut and hindgut endoderm (liver, lung, pancreas, stomach, intestine, prostate and bladder). Foxa2 and Foxa1 were also detected in organs deriving from ectodermal (several brain structures and olfactory epithelium) and mesodermal origins (kidney, vagina and uterus, seminal and coagulating glands) during development. Colocalization and distinct sites of expression of Foxa1 and Foxa2 indicate unique as well as overlapping roles of Foxa1 or Foxa2 during morphogenesis and in the function of different adult organs. (C) 2004 Elsevier B.V. All rights reserved.