Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages
Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages
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DOI：
10.1128/mcb.25.10.3896-3905.2005
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发表时间：
2005-05-01
影响因子：
5.3
通讯作者：
Rajewsky, K
中科院分区：
文献类型：
--
作者：
Oberdoerffer, P;Kanellopoulou, C;Rajewsky, K
RNA 干扰 (RNAi) 是一种自然发生的转录后基因沉默机制，已被用作多种生物体功能丧失研究的遗传工具。它比传统的基因靶向应用更广泛，并且允许用单个转基因同时灭活多个同源基因。最近，RNAi 已被用于小鼠的条件性和常规基因失活。与基因靶向不同，RNAi 是一个动态过程，其效率可能因细胞类型和整个发育过程而异。在这里，我们证明 RNAi 可用于在小鼠中有条件地靶向 bcl-2 家族基因 bfl-1/A1 的三种单独编码的亚型。基因失活的程度因不同细胞类型而异，并且在成熟淋巴细胞中效率最低。我们的数据表明，RNAi 还受到小干扰 RNA-mRNA 化学计量以外的因素的影响。
RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is more widely applicable than classical gene targeting and allows for the simultaneous inactivation of several homologous genes with a single transgene. Recently, RNAi has been used for conditional and conventional gene inactivation in mice. Unlike gene targeting, RNAi is a dynamic process, and its efficiency may vary both between cell types and throughout development. Here we demonstrate that RNAi can be used to target three separately encoded isoforms of the bcl-2 family gene bfl-1/A1 in a conditional manner in mice. The extent of gene inactivation varies between different cell types and is least efficient in mature lymphocytes. Our data suggest that RNAi is affected by factors beyond small interfering RNA-mRNA stoichiometry.