AVR2 Targets BSL Family Members, Which Act as Susceptibility Factors to Suppress Host Immunity
AVR2 Targets BSL Family Members, Which Act as Susceptibility Factors to Suppress Host Immunity
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DOI：
10.1104/pp.18.01143
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发表时间：
2019-05-01
期刊：
影响因子：
7.4
通讯作者：
Birch, Paul R. J.
中科院分区：
文献类型：
--
作者：
Turnbull, Dionne;Wang, Haixia;Birch, Paul R. J.
为了成为成功的植物病原体，微生物使用“效应蛋白”来操纵宿主功能以使其受益。识别宿主靶的效应蛋白，并表征其在感染过程中的作用，使我们能够更好地了解植物-病原体相互作用和植物免疫系统。酵母双杂交分析和免疫共沉淀用于证明致病疫霉效应子AVIRULENCE 2（PiAVR 2）与来自马铃薯（Solanum tuberosum）的所有三个BRI 1-SUPPLIOR 1-like（BSL）家族成员相互作用。BSL 1、BSL 2和BSL 3的瞬时表达增强了致病疫霉叶感染。BSL 1和BSL 3抑制INFESTIN 1诱导素触发的细胞死亡，表明它们负调节免疫。病毒诱导的基因沉默研究表明，BSL 2和BSL 3是BSL 1稳定性所需的，并表明在BSL沉默的植物中基础免疫水平增加。BSL家族成员的免疫抑制依赖于油菜素类固醇响应性宿主转录因子CIB 1/HBI 1样1。致病疫霉效应子PiAVR 2靶向作物S中的所有三个BSL家族成员。马铃薯已知这些磷酸酶在促进生长的油菜素类固醇信号传导中起作用，它们都支持致病疫霉的毒力，因此可以被认为是晚疫病感染的易感因子。
To be successful plant pathogens, microbes use "effector proteins" to manipulate host functions to their benefit. Identifying host targets of effector proteins and characterizing their role in the infection process allow us to better understand plant-pathogen interactions and the plant immune system. Yeast two-hybrid analysis and coimmunoprecipitation were used to demonstrate that the Phytophthora infestans effector AVIRULENCE 2 (PiAVR2) interacts with all three BRI1-SUPPRESSOR1-like (BSL) family members from potato (Solanum tuberosum). Transient expression of BSL1, BSL2, and BSL3 enhanced P. infestans leaf infection. BSL1 and BSL3 suppressed INFESTIN 1 elicitin-triggered cell death, showing that they negatively regulate immunity. Virus-induced gene silencing studies revealed that BSL2 and BSL3 are required for BSL1 stability and show that basal levels of immunity are increased in BSL-silenced plants. Immune suppression by BSL family members is dependent on the brassinosteroid-responsive host transcription factor CIB1/HBI1-like 1. The P. infestans effector PiAVR2 targets all three BSL family members in the crop plant S. tuberosum. These phosphatases, known for their role in growth-promoting brassinosteroid signaling, all support P. infestans virulence and thus can be regarded as susceptibility factors in late blight infection.