Salmonella enterica highly expressed genes are disease specific
Salmonella enterica highly expressed genes are disease specific
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DOI：
10.1128/iai.74.3.1649-1660.2006
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发表时间：
2006-03-01
影响因子：
3.1
通讯作者：
Bumann, D
中科院分区：
文献类型：
--
作者：
Rollenhagen, C;Bumann, D
在体外肉汤培养期间，细菌基因表达通常由参与蛋白质生物合成、成熟和折叠的高表达因子主导，但尚不清楚这是否也适用于自然环境中的条件。在这里，我们使用了一个启动子陷阱的策略，一个不稳定的绿色荧光蛋白报告，可以在感染的小鼠组织中检测到，以确定21沙门氏菌启动子在小鼠肠炎模型的高水平的活动。然后，我们在肠炎和鼠伤寒模型中测量了这些和31个先前鉴定的沙门氏菌启动子的活性。令人惊讶的是，数据显示，而不是蛋白质生物合成基因，疾病特异性基因，如沙门氏菌致病岛1（SPI-1）相关基因和参与厌氧呼吸（肠炎）或SPI-2相关基因和基因的PhoP调节子（伤寒），分别占主导地位的沙门氏菌体内基因表达。高表达基因的整体功能概况表明，肠炎期间的营养利用或伤寒期间对抗宿主的主要转录活动发生了显着变化。在已鉴定的基因中，大部分已知和新的必需毒力因子表明感染期间的高表达水平可能与功能相关性相关。
During in vitro broth culture, bacterial gene expression is typically dominated by highly expressed factors involved in protein biosynthesis, maturation, and folding, but it is unclear if this also applies to conditions in natural environments. Here, we used a promoter trap strategy with an unstable green fluorescent protein reporter that can be detected in infected mouse tissues to identify 21 Salmonella enterica promoters with high levels of activity in a mouse enteritis model. We then measured the activities of these and 31 previously identified Salmonella promoters in both the enteritis and a murine typhoid fever model. Surprisingly, the data reveal that instead of protein biosynthesis genes, disease-specific genes such as Salmonella pathogenicity island 1 (SPI-1)-associated genes and genes involved in anaerobic respiration (enteritis) or SPI-2-associated genes and genes of the PhoP regulon (typhoid fever), respectively, dominate Salmonella in vivo gene expression. The overall functional profile of highly expressed genes suggests a marked shift in major transcriptional activities to nutrient utilization during enteritis or to fighting against the host during typhoid fever. The large proportion of known and novel essential virulence factors among the identified genes suggests that high expression levels during infection may correlate with functional relevance.