Real-time label-free immunoassay of interferon-gamma and prostate-specific antigen using a Fiber-Optic Localized Surface Plasmon Resonance sensor
Real-time label-free immunoassay of interferon-gamma and prostate-specific antigen using a Fiber-Optic Localized Surface Plasmon Resonance sensor
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DOI：
10.1016/j.bios.2012.08.013
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发表时间：
2013-01-15
影响因子：
12.6
通讯作者：
Lee, Seung-Ki
中科院分区：
文献类型：
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作者：
Jeong, Hyeon-Ho;Erdene, Norov;Lee, Seung-Ki
光纤局域表面等离子共振 (FO LSPR) 传感器是在光纤的扁平端面上使用球形金纳米粒子 (Au NP) 制造的。 Au NPs很容易通过Turkevich方法合成，并通过使用自组装单层（SAM）固定在光纤的端面上。为了考察其作为无标记免疫分析生物传感器应用的可能性，将制作的FO LSPR传感器用于检测干扰素-γ（IFN-γ）的抗体-抗原反应，检测限（LOD）约为2 pg/ml。在此，抗体和牛血清白蛋白（BSA）通过物理吸附固定在金纳米粒子上。此外，FO LSPR传感器用于检测前列腺特异性抗原(PSA)，LOD低于1pg/ml。所制备的FO LSPR传感器可用于实时无标记免疫分析，具有检测时间快、分辨率高和灵敏度高的特点。此外，所提出的传感器平台具有成本低、光学设置简单、遥感、制造简单、实时检测、样品量少以及在体内检测系统中的潜在应用等优点。 (C) 2012 Elsevier B.V. 保留所有权利。
A Fiber-Optic Localized Surface Plasmon Resonance (FO LSPR) sensor was fabricated using spherical gold nanoparticles (Au NPs) on a flattened end-face of the optical fiber. The Au NPs were easily synthesized by the Turkevich method and were immobilized on the end-face of the optical fiber by using a self-assembled monolayer (SAM). In order to examine the possibility of its application as a biosensor for label-free immunoassays, the fabricated FO LSPR sensor was used for the detection of the antibody-antigen reaction of interferon-gamma (IFN-gamma) and the limit of detection (LOD) was approximately 2 pg/ml. Herein, The antibodies and bovine serum albumins (BSAs) were immobilized on the Au NPs by physisorption. Also, the FO LSPR sensor was used for the detection of a prostate-specific antigen (PSA) and the LOD was 1 pg/ml below. The fabricated FO LSPR sensor can be used for real-time label-free immunoassay having fast detection time, high resolution and sensitivity. In addition, the proposed sensor platform has the advantages of low cost, simple optical setup, remote sensing, simple fabrication, real-time detection, low sample volume, and potential application to in-vivo detection systems. (C) 2012 Elsevier B.V. All rights reserved.