COMPARISON OF THE AMINO-ACID-SEQUENCES OF THE MAJOR NEUTRALIZATION PROTEIN OF 4 HUMAN ROTAVIRUS SEROTYPES
COMPARISON OF THE AMINO-ACID-SEQUENCES OF THE MAJOR NEUTRALIZATION PROTEIN OF 4 HUMAN ROTAVIRUS SEROTYPES
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DOI：
10.1016/0042-6822(87)90181-4
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发表时间：
1987-11-01
期刊：
影响因子：
3.7
通讯作者：
FLORES, J
中科院分区：
文献类型：
--
作者：
GREEN, KY;MIDTHUN, K;FLORES, J
我们对代表血清型 1、2、3 或 4 的八种人轮状病毒株的主要中和蛋白 (VP7) 的编码基因进行了测序。此外，还对恒河猴轮状病毒疫苗株 MMU18006（血清型 3）的相应基因进行了测序。对推导的氨基酸序列的比较分析显示，定义四种不同轮状病毒血清型的 VP7 蛋白总体存在 15-29% 的差异，并证实存在六个离散的聚类序列差异区域（氨基酸 39-50、87-101、120-130、143-152、208-221 和 233-242）。当对属于相同血清型的轮状病毒的相同区域进行比较时，检测到高度的同源性（91-99%）。这些观察表明，轮状病毒之间血清型的差异是 VP7 基因的几个离散区域中高度序列差异的结果，并且这些区域在给定血清型内高度保守。
We sequenced the gene coding for the major neutralizing protein (VP7) from eight human rotavirus strains representing serotype 1, 2, 3, or 4. In addition, the corresponding gene of the rhesus rotavirus vaccine strain MMU18006 (serotype 3) was sequenced. Comparative analyses of their deduced amino acid sequences revealed an overall 15-29% divergence in the VP7 proteins that define four different rotavirus serotypes and confirmed the presence of six discrete regions of clustered sequence divergence (amino acids 39-50, 87-101, 120-130, 143-152, 208-221, and 233-242). When the same regions were compared among rotaviruses belonging to the same serotype, a high degree of homology (91-99%) was detected. These observations indicate that differences in the serotype specifically among rotaviruses are the result of a high degree of sequence divergence in several discrete regions of the VP7 gene and that these regions are highly conserved within a given serotype.