Neurotensin-like immunoreactivity in the preganglionic sympathetic nerves and in the adrenal medulla of the cat.
Neurotensin-like immunoreactivity in the preganglionic sympathetic nerves and in the adrenal medulla of the cat.
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猫的节前交感神经和肾上腺髓质中的神经降压素样免疫反应性。
DOI：
10.1111/j.1748-1716.1982.tb06965.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
M. Goldstein
中科院分区：
文献类型：
--
作者：
J. Lundberg;Å. Rökaeus;T. Hökfelt;S. Rosell;M. Brown;M. Goldstein
Carraway和Leeman分离并鉴定了神经降压素，并确定了其在脑和肠中的存在（参见Leeman等人，1977）。神经降压素的细胞分布首先由Orci等人描述。（1976年）。他们在小肠粘膜的特定内分泌细胞中发现了这种肽。随后，在中枢神经系统的神经中也可以证明神经降压素（Uhl等，1978）。还描述了大鼠胃肠道中的神经降压素免疫反应性神经（Lundberg等人，1980）。在目前的简短通讯的周围神经系统和肾上腺的猫进行了化学分析和放射免疫分析方面的分布神经降压素样免疫反应。用10%福尔马林灌注两种性别的成年猫。切下各种外周组织，浸入相同的固定液中，冲洗并处理用于间接免疫荧光技术。在一些猫结扎腹侧和背侧脊髓根在胸中段水平和内脏，坐骨神经和迷走神经灌注前24小时。如前所述（Rokaeus 1981），通过放射免疫测定法分析各种组织（见下文）。简言之，在pH 4.0的0.5 M醋酸盐缓冲液中提取组织，煮沸并超声处理。离心后，测定上清液。对于免疫组织化学研究，使用抗体NT 1-1 - 1（小林等人，1977），并且在放射免疫测定中使用抗体0-7701（Rokaeus 1981）。对于免疫组织化学中的对照，用过量的神经降压素（50 μ g肽/ml抗血清1：10稀释）预处理抗血清。如前所述（Goldstein等，1974）制备了儿茶酚胺合成酶多巴胺P-羟化酶（DBH）和苯乙醇胺N-甲基转移酶（PNMT）的抗血清。正常兔血清用作DBH和PNMT抗血清的对照血清。免疫组织化学在神经系统中，神经降压素样免疫反应主要见于三个不同的部位：交感神经节、胃肠道和节前交感神经干。在交感神经节中，观察到不同数量的神经降压素免疫反应纤维周围的DBH阳性的主神经节细胞，主要是在斑片状的方式。在星状神经节（图10）和交感神经链的L7神经节中观察到密集或中等密集的免疫反应神经网络，具有明显的区域定位。此外，腹腔神经节含有中等密度的神经丛，但分布更均匀。上级颈神经节以及上级和肠系膜下神经节仅含有少量纤维。神经降压素免疫反应纤维不存在周围的小强荧光（SIF）细胞。在副交感神经节，如睫状神经节、耳神经节和盆丛中尚未发现神经降压素免疫反应纤维。此外，脊神经节、三叉神经节和结状神经节似乎缺乏神经降压素免疫反应纤维和胞体。到目前为止，在外周组织如唾液腺、虹膜、心脏、肺和泌尿生殖道中没有观察到神经降压素免疫反应纤维。在胃肠道神经紧张素样免疫反应主要发现在内分泌细胞，但也看到了少量的免疫反应纤维的肌间神经丛，主要是在心室。在胃肠道的这一部分肌间神经丛中偶尔也可见到免疫反应性神经元胞体。结论：神经紧张素样免疫反应阳性神经元的表达与周围神经损伤密切相关。
Carraway & Leeman isolated and characterized neurotensin and established its presence in brain and intestine (see Leeman et al. 1977). The cellular distribution of neurotensin was first described by Orci et al.(1976). They found this peptide in a specific endocrine cell in the small intestinal mucosa. Subsequently neurotensin could also be demonstrated in nerves in the central nervous system (Uhl et al. 1978). Neurotensin immunoreactive nerves have also been described in the gastrointestinal tract of the rat (Lundberg et al. 1980). In the present short communication the peripheral nervous system and adrenal gland of the cat were analysed immunohistochemically and by radioimmunoassay with regard to distribution of neurotensin-like immunoreactivity. Adult cats of both sexes were perfused with 10% formalin. Various peripheral tissues were dissected out, immersed in the same fixative, rinsed and processed for indirect immunofluorescence technique. In some cats ligations were performed on ventral and dorsal spinal roots at the mid-thoracic level and on the splanchnic, sciatic and vagus nerves 24 h before perfusion. Various tissues (see below) were analysed by radioimmunoassay as described previously (Rokaeus 1981). Briefly, the tissues were extracted in 0.5 M acetate buffer at pH 4.0, boiled, and sonicated. Following centrifugation the supernatant was assayed. For the immunohistochemical studies the antibody NT 1-1 1 (Kobayashi et al. 1977) was used and in the radioimmunoassay antibody 0-7701 (Rokaeus 1981). For control in immunohistochemistry the antiserum was pretreated with an excess of neurotensin (50 pg peptidelml antiserum diluted 1: 10). Antisera to the catecholamine synthesizing enzymes dopamine P-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) were prepared as described earlier (Goldstein et al. 1974). Normal rabbit serum served as control sera to DBH and PNMT antisera. lmmunohistochemistry. In the nervous system neurotensin-like irnmunoreactivity was mainly found in three different localizations: Sympathetic ganglia, gastrointestinal tract and in preganglionic sympathetic nerve trunks. In sympathetic ganglia varying amounts of neurotensin immunoreactive fibres were observed around the DBH-positive principal ganglion cells, mostly in a patchy fashion. Dense or moderately dense networks of immunoreactive nerves were seen in the stellate ganglion (Fig. lo) and in the L7 ganglion of the sympathetic chain with a marked regional localization. Also the celiac ganglion contained a moderately dense plexus but more uniformly distributed. The superior cervical as well as the superior and inferior mesenteric ganglia contained only few fibres. The neurotensin immunoreactive fibres were not present around small intensely fluorescent (SIF) cells. No neurotensin immunoreactive fibres have so far been observed in parasympathetic ganglia, such as ganglion ciliare, ganglion oticum and plexus pelvicus. Furthermore, the spinal, trigeminal and nodose ganglia seemed to lack neurotensin immunoreactive fibres and cell bodies. So far, no neurotensin immunoreactive fibres have been observed in peripheral tissues such as the salivary glands, iris, heart, lung and urogenital tract. In the gastrointestinal tract neurotensin-like immunoreactivity was mainly found in endocrine cells, but low numbers of immunoreactive fibres were also seen in the myenteric plexus, mainly in the ventricle. Occasionally immunoreactive neuronal cell bodies could also be seen in the myenteric plexus in this part of the gastrointestinal tract. After ligation of peripheral nerves accumulation of neurotensin-like immunoreactivity was …