The mRNA expression of neurocan, a brain-specific chondroitin sulfate proteoglycan, in neoplastic mammary glands in mice
The mRNA expression of neurocan, a brain-specific chondroitin sulfate proteoglycan, in neoplastic mammary glands in mice
复制标题
DOI：
10.2108/zsj.13.665
复制
发表时间：
1996-10-01
影响因子：
0.9
通讯作者：
Nagasawa, H
中科院分区：
文献类型：
--
作者：
Harigaya, T;Ogawa, H;Nagasawa, H
在小鼠转化生长因子α（TGF α）基因在乳腺肿瘤中表达的实验中，除了在四种品系的小鼠中预测的大小之外，还检测到使用小鼠TGF α引物的逆转录-聚合酶链反应（RT-PCR）的各种大小的扩增产物。在进一步分析小鼠乳腺肿瘤RT-PCR产物的过程中，用小鼠TGF α引物进行RT-PCR反应后，通过克隆和核苷酸序列分析，在SHN小鼠乳腺肿瘤中检测到neurocan基因的转录本。在扩增的527 bp的cDNA序列中，连续246 bp的5 ′端核苷酸序列与神经组织中表达的硫酸软骨素蛋白多糖之一的小鼠神经多糖cDNA序列的中间部分完全相同。
In the experiment of mouse transforming growth factor alpha (TGF alpha) gene expression in mammary tumors, various sizes of amplified products by reverse transcriptase-polymerase chain reaction (RT-PCR) using mouse TGF alpha primers were detected in addition to a predicted size in four strains of mice. During the further analysis of these RT-PCR products in mouse mammary tumors, the transcript of neurocan gene was detected in the mammary tumor from SHN mice by the cloning and nucleotide sequence analysis after RT-PCR reaction using mouse TGF alpha primers. The 5'-nucleotide sequence of sequential 246bp in the amplified cDNA of 527bp was completely identical to a middle part of mouse neurocan cDNA sequence, one of the chondroitin-sulfate proteoglycan expressed in the nervous tissue.