Changes in morphology of retinal ganglion cells with eccentricity in retinal degeneration
Changes in morphology of retinal ganglion cells with eccentricity in retinal degeneration
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DOI：
10.1007/s00441-015-2337-y
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发表时间：
2016-05-01
影响因子：
3.6
通讯作者：
Fletcher, E. L.
中科院分区：
文献类型：
--
作者：
Anderson, E. E.;Greferath, U.;Fletcher, E. L.
神经节细胞是视网膜的输出神经元，在遗传性视网膜变性中光感受器死亡后发生的细微可塑性变化中，神经节细胞被认为是重塑的。我们研究了视网膜偏心对感光细胞丧失后的解剖重建和神经节细胞形态的影响。以磷酸二酯酶6β亚基突变的RD1小鼠作为视网膜变性模型，通过连续切片免疫细胞化学方法检测大体重塑事件。来自Rd1-Thy1和对照Thy1小鼠的视网膜整体含有标记神经节细胞亚群的荧光蛋白，并在11个月大时进行免疫组织化学处理。根据神经节细胞的胞体大小、树突野大小和树突分支模式对神经节细胞进行分类，并对其树突野的长度、面积和分支点的数量进行分析。总体而言，与外围视网膜相比，中央视网膜的重塑更多。此外，位于视网膜中央区的A2、B1、C1型和D型神经节细胞的大小和复杂性均减小。我们认为，神经节细胞形态的变化与这些区域的重塑事件有关，并影响退化视网膜的视网膜回路功能。
Ganglion cells are the output neurons of the retina and are known to remodel during the subtle plasticity changes that occur following the death of photoreceptors in inherited retinal degeneration. We examine the influence of retinal eccentricity on anatomical remodelling and ganglion cell morphology well after photoreceptor loss. Rd1 mice that have a mutation in the beta subunit of phosphodiesterase 6 were used as a model of retinal degeneration and gross remodelling events were examined by processing serial sections for immunocytochemistry. Retinal wholemounts from rd1-Thy1 and control Thy1 mice that contained a fluorescent protein labelling a subset of ganglion cells were processed for immunohistochemistry at 11 months of age. Ganglion cells were classified based on their soma size, dendritic field size and dendritic branching pattern and their dendritic fields were analysed for their length, area and quantity of branching points. Overall, more remodelling was found in the central compared with the peripheral retina. In addition, the size and complexity of A2, B1, C1 and D type ganglion cells located in the central region of the retina decreased. We propose that the changes in ganglion cell morphology are correlated with remodelling events in these regions and impact the function of retinal circuitry in the degenerated retina.