TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data.

TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data.
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TRUST 4:从批量和单细胞RNA-seq数据重建免疫库。

DOI:
10.1038/s41592-021-01142-2
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发表时间:
2021-06
期刊:
影响因子:
48
通讯作者:
Liu, X. Shirley
Liu, X. Shirley
中科院分区:
生物学1区
文献类型:
--
作者:
Song, Li;Cohen, David;Ouyang, Zhangyi;Cao, Yang;Hu, Xihao;Liu, X. Shirley

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我们介绍了TRUST 4开源算法,用于从RNA-seq数据重建αβ/γδ T细胞和B细胞中的免疫受体库。与竞争方法相比,TRUST 4支持FASTQ和BAM格式,在组装更长甚至全长受体库方面更快,更灵敏。TRUST 4还可以调用来自单细胞RNA-seq(scRNA-seq)数据的库序列,而无需V(D)J富集,并且与SMART-seq和5'10 X Genomics平台兼容。
We introduce the TRUST4 open-source algorithm for reconstructing immune receptor repertoires in αβ/γδ T-cells and B-cells from RNA-seq data. Compared to competing methods, TRUST4 supports both FASTQ and BAM formats, is faster and more sensitive in assembling longer, even full-length, receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5’ 10X Genomics platforms.
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