TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data.
TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data.
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TRUST 4:从批量和单细胞RNA-seq数据重建免疫库。
DOI:
10.1038/s41592-021-01142-2
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发表时间:
2021-06
期刊:
影响因子:
48
通讯作者:
Liu, X. Shirley
中科院分区:
文献类型:
--
作者:
Song, Li;Cohen, David;Ouyang, Zhangyi;Cao, Yang;Hu, Xihao;Liu, X. Shirley
We introduce the TRUST4 open-source algorithm for reconstructing immune receptor repertoires in αβ/γδ T-cells and B-cells from RNA-seq data. Compared to competing methods, TRUST4 supports both FASTQ and BAM formats, is faster and more sensitive in assembling longer, even full-length, receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5’ 10X Genomics platforms.
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