Comparison of rhinovirus A infection in human primary epithelial and HeLa cells
Comparison of rhinovirus A infection in human primary epithelial and HeLa cells
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DOI：
10.1099/vir.0.031302-0
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发表时间：
2011-11-01
影响因子：
3.8
通讯作者：
Palmenberg, A. C.
中科院分区：
文献类型：
--
作者：
Amineva, S. P.;Aminev, A. G.;Palmenberg, A. C.
HeLa 细胞用于研究许多不同病毒的生命周期，包括小RNA病毒科中的人类鼻病毒 (HRV)。尽管 HRV 的天然靶标是人支气管上皮细胞 (hBE)，但相对于 He La 细胞，获得和维持相关的原代细胞培养物通常更困难。鉴于 HRV 现在被确定为人类哮喘恶化的主要原因，记录从 He La 细胞学到的病毒生物学有多少对于天然原代细胞也很常见就变得很重要。当使用 A01a 病毒在匹配感染中直接进行比较时，hBE 和 He La 细胞中的 RNA 复制动力学、病毒蛋白的合成和加工以及关键非结构蛋白的一般亚细胞定位相似。病毒诱导的宿主细胞过程（例如核质运输）的关闭也具有可比性。
HeLa cells are used to study the life cycles of many different viruses, including the human rhinoviruses (HRV) in the family Picomaviridae. Although the natural targets of HRV are human bronchial epithelial cells (hBE), it is generally more difficult to obtain and maintain the relevant primary cell cultures, relative to He La cells. Given that the HRV are now identified as a major cause of human asthma exacerbations, it becomes important to document how much of the virus biology learned from He La cells is common also to natural primary cells. When compared directly in matched infections using A01a virus, the kinetics of RNA replication, the synthesis and processing of viral proteins and the general subcellular localization of key non-structural proteins were resembled in hBE and He La cells. Viral-induced shutoff of host cell processes (e.g. nucleo-cytoplasmic trafficking) was also comparable.