Temporal Variations in the Gut Microbiota of the Globally Endangered Sichuan Partridge (Arborophila rufipectus): Implications for Adaptation to Seasonal Dietary Change and Conservation
Temporal Variations in the Gut Microbiota of the Globally Endangered Sichuan Partridge (Arborophila rufipectus): Implications for Adaptation to Seasonal Dietary Change and Conservation
复制标题
DOI：
10.1128/aem.00747-23
复制
发表时间：
2023-06
影响因子：
4.4
通讯作者：
Keyi Tang;Ling Tao;Yufeng Wang;Qiong Wang;Changkun Fu;Benping Chen;Zhengwang Zhang;Yiqiang Fu-Yiqiang
中科院分区：
文献类型：
--
作者：
Keyi Tang;Ling Tao;Yufeng Wang;Qiong Wang;Changkun Fu;Benping Chen;Zhengwang Zhang;Yiqiang Fu-Yiqiang
描述不同季节野生鸟类的肠道微生物将揭示它们的年度生命周期。由于采样困难和缺乏详细的饮食信息，关于肠道微生物群如何适应野生鸟类季节性饮食变化的研究很少。摘要宿主相关微生物群通过帮助消化、代谢、营养、生理、免疫功能和病原体抵抗力来影响宿主健康。虽然越来越多的研究已经调查了鸟类微生物组，但缺乏对野生鸟类，特别是濒危野鸡肠道微生物的研究。由于很难表征膳食组成的动态，特别是在杂食动物中，鸟类的肠道微生物对季节性膳食变化的反应仍然知之甚少。川雉（Arborophila rufipectus）是我国西南山区特有的一种数量较少的濒危雉类。本文采用16 S rRNA序列分析和Tax 4Fun技术，对四川鹧鸪繁殖、繁殖后游走和越冬3个关键时期肠道菌群的结构和功能进行了研究。结果表明，全年微生物群落以厚壁菌门、变形菌门、放线菌门和蓝藻门为主。肠道微生物的多样性最高的繁殖后流浪和越冬期间最低。季节性的饮食变化和肠道微生物群落的重组发生一致。肠道微生物群的组成、多样性和功能表现出与饮食相关的变化，这可能有助于宿主适应不同的饮食以应对环境变化。此外，检测到28个潜在的致病菌属，它们的组成在三个时期之间存在显著差异。对野生鸟类肠道微生物群动态的研究增强了我们对鸟类年生命周期中饮食微生物群关联的理解，有助于这种濒危鸟类的综合保护。重要性描述野生鸟类不同季节的肠道微生物群落特征将有助于了解它们的年度生命周期。由于采样困难和缺乏详细的饮食信息，关于肠道微生物群如何适应野生鸟类季节性饮食变化的研究很少。基于更详细的食物组成，我们发现了四川鹧鸪肠道菌群的季节性重塑模式，与它们的季节性饮食变化相对应。饮食和肠道微生物群的变化可能促进了这种濒危野鸡的饮食生态位的多样性，揭示了一个季节性的饮食微生物群协会在三个时期的年度周期。此外，鉴定各种潜在致病细菌属有助于管理四川鹧鸪的健康和提高其存活率。将微生物组研究纳入濒危物种保护，有助于我们全面了解野生鸟类的饮食-宿主-微生物组关系，并改进保护实践。
Characterizing the gut microbiotas of wild birds across seasons will shed light on their annual life cycle. Due to sampling difficulties and the lack of detailed dietary information, studies on how the gut microbiota adapts to seasonal dietary changes of wild birds are scarce. ABSTRACT Host-associated microbiotas are known to influence host health by aiding digestion, metabolism, nutrition, physiology, immune function, and pathogen resistance. Although an increasing number of studies have investigated the avian microbiome, there is a lack of research on the gut microbiotas of wild birds, especially endangered pheasants. Owing to the difficulty of characterizing the dynamics of dietary composition, especially in omnivores, how the gut microbiotas of birds respond to seasonal dietary changes remains poorly understood. The Sichuan partridge (Arborophila rufipectus) is an endangered pheasant species with a small population endemic to the mountains of southwest China. Here, 16S rRNA sequencing and Tax4Fun were used to characterize and compare community structure and functions of the gut microbiota in the Sichuan partridges across three critical periods of their annual life cycle (breeding, postbreeding wandering, and overwintering). We found that the microbial communities were dominated by Firmicutes, Proteobacteria, Actinobacteria, and Cyanobacteria throughout the year. Diversity of the gut microbiotas was highest during postbreeding wandering and lowest during the overwintering periods. Seasonal dietary changes and reassembly of the gut microbial community occurred consistently. Composition, diversity, and functions of the gut microbiota exhibited diet-associated variations, which might facilitate host adaptation to diverse diets in response to environmental shifts. Moreover, 28 potential pathogenic genera were detected, and their composition differed significantly between the three periods. Investigation of the wild bird gut microbiota dynamics has enhanced our understanding of diet-microbiota associations over the annual life cycle of birds, aiding in the integrative conservation of this endangered bird. IMPORTANCE Characterizing the gut microbiotas of wild birds across seasons will shed light on their annual life cycle. Due to sampling difficulties and the lack of detailed dietary information, studies on how the gut microbiota adapts to seasonal dietary changes of wild birds are scarce. Based on more detailed dietary composition, we found a seasonal reshaping pattern of the gut microbiota of Sichuan partridges corresponding to their seasonal dietary changes. The variation in diet and gut microbiota potentially facilitated the diversity of dietary niches of this endangered pheasant, revealing a seasonal diet-microbiota association across the three periods of the annual cycle. In addition, identifying a variety of potentially pathogenic bacterial genera aids in managing the health and improving survival of Sichuan partridges. Incorporation of microbiome research in the conservation of endangered species contributes to our comprehensive understanding the diet-host-microbiota relationship in wild birds and refinement of conservation practices.