DNA N6-Adenine Methylation in Arabidopsis thaliana
DNA N6-Adenine Methylation in Arabidopsis thaliana
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DOI：
10.1016/j.devcel.2018.03.012
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发表时间：
2018-05-07
影响因子：
11.8
通讯作者：
Yu, Hao
中科院分区：
文献类型：
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作者：
Liang, Zhe;Shen, Lisha;Yu, Hao
最近发现，在一些单细胞和多细胞真核生物中，N-6-腺嘌呤（6 mA）上的DNA甲基化是一种潜在的表观遗传标记。然而，它在陆地植物中的分布模式和潜在功能，这是大多数生态系统的初级生产者，仍然在很大程度上未知。在这里，我们报告了使用单分子实时测序以单核苷酸分辨率对处于不同发育阶段的拟南芥基因组中的6 mA位点进行的全局分析。6 mA位点广泛分布在拟南芥基因组中，并在着丝粒周围异染色质区域富集。6 mA在基因体中的发生频率高于基因间区域。对6 mA甲基化组和RNA测序数据的分析表明，6 mA频率与拟南芥基因表达水平和从营养生长到生殖生长的转变呈正相关。我们的研究结果揭示了6 mA作为一个DNA标记与活跃表达的基因在拟南芥，这表明6 mA作为一个迄今为止未知的表观遗传标记在陆地植物。
DNA methylation on N-6-adenine (6mA) has recently been found to be a potentially epigenetic mark in several unicellular and multicellular eukaryotes. However, its distribution patterns and potential functions in land plants, which are primary producers for most ecosystems, remain largely unknown. Here we report global profiling of 6mA sites at single-nucleotide resolution in the genome of Arabidopsis thaliana at different developmental stages using single-molecule real-time sequencing. 6mA sites are widely distributed across the Arabidopsis genome and enriched over the pericentromeric heterochromatin regions. 6mA occurs more frequently in gene bodies than intergenic regions. Analysis of 6mA methylomes and RNA sequencing data demonstrates that 6mA frequency positively correlates with the gene expression level and the transition from vegetative to reproductive growth in Arabidopsis. Our results uncover 6mA as a DNA mark associated with actively expressed genes in Arabidopsis, suggesting that 6mA serves as a hitherto unknown epigenetic mark in land plants.