Fine-scale spatial genetic structure of an endangered marsh herb, Caldesia grandis (Alismataceae).
Fine-scale spatial genetic structure of an endangered marsh herb, Caldesia grandis (Alismataceae).
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DOI：
10.4238/2012.may.18.10
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发表时间：
2012-08
期刊：
影响因子：
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通讯作者：
Xiao-Li Yue;J.-M. Chen;Y.-H. Guo;Q-F Wang
中科院分区：
文献类型：
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作者：
Xiao-Li Yue;J.-M. Chen;Y.-H. Guo;Q-F Wang
濒临灭绝的沼泽草本植物-研究了三个群体的遗传变异的空间结构。利用RAPD标记和空间自相关分析，基于等距离间隔法，从中国湖南和云南两省采集的4个斑块中，共采集了157个个体。在7个选择性引物产生的多态性条带中，频率在20 - 80%之间的多态性条带用于计算每个斑块的Moran's I空间自相关系数。结果表明，在北海种群（BH-1和BH-2）和怪湖种群（GH-1）的3个斑块中，遗传变异具有明显的空间结构。grandis（在短距离类内具有显著的正自相关）。相反，在狼盘湖（LPH）种群（斑块LPH-1）的遗传变异被发现是随机分布的。不同的空间分布格局可能是由于环境的差异。这些结果对该物种的保护和管理，特别是迁地保护的采样策略具有一定的意义。
The endangered marsh herb, Caldesia grandis, is native to China. We investigated the spatial structure of the genetic variation of three populations of C. grandis using RAPD markers and spatial autocorrelation analysis, based on the method of equal distance interval. A total of 157 individuals were sampled from four patches collected from the region of Hunan and Yunnan Provinces, China. Among the polymorphic bands generated by seven selective primers, polymorphic bands with frequencies ranging from 20 to 80% were used to calculate Moran's I spatial autocorrelation coefficient for each patch. We found significant spatial structure of genetic variation in the three patches in Bei Hai (BH) (patches BH-1 and BH-2) and Guai Hu (GH) (patch GH-1) populations of C. grandis (with significant positive autocorrelation within the short distance class). In contrast, the genetic variation in the Lang Pan Hu (LPH) population (patch LPH-1) was found to be randomly distributed. The different spatial distribution patterns may be attributed to environment differences. These results have implications for the conservation and management of this species, especially for sampling strategies for ex situ conservation.