A NEW ASSAY SUITABLE FOR ENUMERATION OF MURINE PROGENITORS OF GRANULO-MONOCYTES AND FOR RAPID AUTOMATED ASSESSMENT OF GRANULO-MONOCYTES GROWTH-FACTORS
A NEW ASSAY SUITABLE FOR ENUMERATION OF MURINE PROGENITORS OF GRANULO-MONOCYTES AND FOR RAPID AUTOMATED ASSESSMENT OF GRANULO-MONOCYTES GROWTH-FACTORS
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DOI：
10.1016/0022-1759(84)90096-6
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发表时间：
1984-01-01
影响因子：
2.2
通讯作者：
MARCHAL, G
中科院分区：
文献类型：
--
作者：
LEBASTARD, M;MILON, G;MARCHAL, G
描述了一种简单且可重复的测定法，用于计数小鼠骨髓或脾中存在的粒细胞 (G) 和单核细胞 (M) 的祖细胞。该测定基于在 Terasaki 板的孔中铺板不同稀释度的测试细胞。 7 天后对 G 和 M 存在的阴性和阳性孔进行评分。通过分析无反应微培养物百分比和铺板细胞数量之间的泊松分布关系，获得了 GM 祖细胞频率的最小估计值。这种液体微培养测定具有许多优点，包括能够在克隆水平上直接原位测定不同的酶活性（如非特异性酯酶），以及能够通过测量增殖反应细胞的 [3H] 胸苷摄取在 3 天内筛选 GM 生长因子的存在。
A simple and reproducible assay is described for enumerating the progenitors of granulocytes (G) and monocytes (M) present in either the bone marrow or spleen of mice. This assay is based upon the plating of different dilutions of test cells in the wells of Terasaki plates. Negative and positive wells for presence of G and M are scored 7 days later. Minimal estimates of GM progenitors frequency are obtained by analysis of the Poisson distribution relationship between the percentage of non-responding microcultures and the numbers of cells plated. This liquid microculture assay offers many advantages including the ability to assay directly in situ at clonal level different enzymatic activities like non-specific esterase and the ability to screen within 3 days the presence of GM growth factors by measuring the [3H]thymidine uptake of proliferating responsive cells.