Telomerase activity and proliferation index in aggressive mature B-cell lymphoma: Comparison to germinal center phenotypic markers
Telomerase activity and proliferation index in aggressive mature B-cell lymphoma: Comparison to germinal center phenotypic markers
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DOI：
10.1016/j.humpath.2003.07.009
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发表时间：
2003-12-01
期刊：
影响因子：
3.3
通讯作者：
Arber, DA
中科院分区：
文献类型：
--
作者：
Chiu, KC;Fine, M;Arber, DA
细胞增殖可以通过各种方法进行评估，包括Ki-67免疫组织化学和端粒酶活性的测量。理论上，这两种方法在特定情况下都会显示出相当的增加。为探讨这两种增殖标志物在侵袭性成熟B细胞淋巴瘤中的关系，对48例侵袭性成熟B细胞淋巴瘤进行研究。研究组包括5例套细胞淋巴瘤（MCL），6例伯基特/伯基特样淋巴瘤（BL），9例滤泡性淋巴瘤，3级（FLC）和28例弥漫性大B细胞淋巴瘤（DLC）。端粒酶活性测定为总产物生成（TPG）单位，并将上述病例的TPG结果与10例反应性滤泡增生的TPG结果进行比较。不同类型淋巴瘤的log TPG（P = 0.0443）和Ki-67（P = 0.0006）差异有统计学意义。Ki-67百分比与TPG评分在FLC中呈正相关（r = 0.9281; P = 0.0003），但在其他类型淋巴瘤中这两个指标之间的相关性较差。聚类分析确定了不同的模式MCL，FLC和BL，但异质模式DLC。因为Ki-67增殖和端粒酶活性的增加在正常的生发中心（GC）的报告，这些测试也被评估作为GC细胞表型的标志物的有用性。在FLC和DLC病例中，GC表型特征与Ki-67百分比增加显著相关（P = 0.0152），但与log TPG增加无关。log TPG升高与CD 10表达相关，Ki-67百分比升高与CD 10和BCL-6表达相关。TPG水平和Ki-67百分比与t（14;18）或BCL-2蛋白表达的存在无关。尽管MCL、FLC和BL的增殖模式相当独特，但这些研究表明，细胞增殖标记物本身不能识别大细胞淋巴瘤的不同亚型。端粒酶活性与Ki-67增殖指数的相关性较差。然而，这些测试确实显示了与GC细胞表型标记物表达的一些相关性。(C)2003年爱思唯尔公司All rights reserved.
Cell proliferation may be evaluated by various methods, including Ki-67 immunohistochemistry and measures of telomerase activity. Both methods would theoretically show comparable increases in a given case. To evaluate the relationship between these 2 markers of proliferation in aggressive mature B-cell lymphomas, 48 cases were studied. The study group included 5 cases of mantle cell lymphoma (MCL); 6 cases of Burkitt's/Burkitt's-like lymphoma (BL); 9 cases of follicular lymphoma, grade 3 (FLC); and 28 cases of diffuse large B-cell lymphoma (DLC). Telomerase activity was measured as total product generated (TPG) units, and TPG results for the aforementioned cases were compared to the TPG results for 10 cases of reactive follicular hyperplasia. An overlap in TPG scores between reactive cases and lymphoma cases was found. Significant differences in both log TPG (P = 0.0443) and Ki-67 (P = 0.0006) were seen in the different lymphoma types. A positive correlation between Ki-67 percentage and TPG score was identified in FLC (r = 0.9281; P = 0.0003), but a poor correlation between these 2 indicators was seen in the other lymphoma types. Cluster analysis identified distinct patterns for MCL, FLC, and BL, but heterogeneous patterns for DLC. Because increases in both Ki-67 proliferation and telomerase activity are reported in normal germinal centers (GCs), these tests were also evaluated for usefulness as markers of a GC cell phenotype. Among the FLC and DLC cases, features of a GC phenotype significantly correlated with increased Ki-67 percentage (P = 0.0152), but not with increased log TPG. An elevated log TPG correlated with CD10 expression, and elevated Ki-67 percentage correlated with both CD10 and BCL-6 expression. TPG level and Ki-67 percentage did not correlate with the presence of t(14;18) or BCL-2 protein expression. Although the proliferation patterns were fairly distinctive for MCL, FLC, and BL, these studies show that markers of cell proliferation do not by themselves,identify distinct subtypes of large cell lymphomas. With the exception of FLC, the tumors exhibited poor correlation between telomerase activity and Ki-67 proliferation index. These tests did show some correlation with expression of GC cell phenotypic markers, however. (C) 2003 Elsevier Inc. All rights reserved.