In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues
In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues
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DOI：
10.1021/ja052911k
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发表时间：
2005-08-17
影响因子：
15
通讯作者：
Burkart, MD
中科院分区：
文献类型：
--
作者：
Clarke, KM;Mercer, AC;Burkart, MD
We demonstrate site-specific reporter labeling of proteins within live cells. By accessing the coenzyme A (CoA) metabolic pathway with a cell-permeable pantetheine analogue, we deliver CoA-bound reporter molecules for post-translational protein modification in vivo. These methods may be applied to natural product pathway manipulation as well as applications in conventional molecular and cellular biology.
