DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells
DREF is Critical for Drosophila Bristle Development by Regulating Endoreplication in Shaft Cells
复制标题
DOI：
10.1247/csf.11004
复制
发表时间：
2011-01-01
影响因子：
1.5
通讯作者：
Yamaguchi, Masamitsu
中科院分区：
文献类型：
--
作者：
Kawamori, Akihito;Yamaguchi, Masamitsu
DREF(DNA复制相关元件结合因子)通过调节多种基因的转录，在体内的复制和增殖中发挥重要作用。然而，由于体内缺乏适当的细胞生物学研究，对DREF在单细胞发育中的作用知之甚少。为了解决这个问题，我们将注意力集中在大型毛类刚毛的发育系统上。通过对单个后盾节(PSC)感觉器官前体(SOP)谱系的细胞谱系分析，结合GAL4/UAS双链RNA靶向表达系统，我们发现DREF在SOP形成过程中的分化过程中没有明显的作用。相反，DREF调节不对称细胞分裂的时间，但可能在不对称细胞分裂的分化过程中没有直接作用。最重要的是，DREF影响轴突细胞和/或突起细胞的复制和生长。进一步的分析表明，DREF对轴细胞的核生长和蛋白质合成是必要的，但不是充分的。最后，可以证明DREF在调控内循环轴细胞中的增殖细胞核抗原转录方面起着关键作用。所有这些结果表明，DREF在毛状体发育的内复制过程中发挥着重要作用，至少部分是通过调节增殖细胞核抗原基因的表达。
DREF (DNA replication-related element-binding factor) plays important roles in replication and proliferation in vivo by regulating transcription of various genes. However, due to a lack of appropriate cell biological studies in vivo, roles of DREF during a single cell development are poorly understood. To address this question, we focused our attention on macrochaetes bristle development system. Utilizing cell lineage analysis focusing on a single posterior scutellar (PSC) macrochaete sensory organ precursor (SOP) lineages in combination with GAL4/UAS targeted expression system for DREF double strand RNA, we revealed that DREF plays no apparent role in differentiation process during SOP formation. Rather, DREF regulates the timing of asymmetric cell division but perhaps plays no direct role in differentiation during asymmetric cell division. Most importantly, DREF affected replication and growth in shaft cells and/or socket cells. Further analysis revealed that DREF is necessary but not sufficient for nuclear growth and protein synthesis in shaft cells. Finally, it could be demonstrated that DREF plays a critical role in regulating pcna transcription in endocycling shaft cells. All these results provide evidence that DREF plays critical roles, especially in endoreplication process of bristle development, at least in part by regulating the pcna gene expression.