Analysis of gene expression following norepinephrine stimulation in the rat pineal gland using DNA microarray technique
Analysis of gene expression following norepinephrine stimulation in the rat pineal gland using DNA microarray technique
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DOI：
10.1034/j.1600-079x.2003.00078.x
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发表时间：
2003-10-01
影响因子：
10.3
通讯作者：
Tosini, G
中科院分区：
文献类型：
--
作者：
Fukuhara, C;Dirden, JC;Tosini, G
一些研究表明，去甲肾上腺素（NE）是调节松果体基因表达的关键神经递质。本研究采用大鼠基因芯片探针阵列技术研究了内斯对大鼠松果体的急性效应。我们的数据表明，NE刺激影响几个基因的调节; 44和29个基因分别上调或下调超过2.5倍。如在以前的研究中所示，芳基烷基胺N-乙酰转移酶，环AMP反应元件调节剂，jun B和c-fos的mRNA水平增加NE刺激。以前没有报道的基因，增加NE刺激在松果体蛋白酪氨酸磷酸酶，核受体，活性和神经递质诱导的早期基因。与上调基因不同，大多数下调基因以前没有报道。NE刺激后，参与代谢的酶和结构蛋白的编码基因减少。研究NE刺激对松果体生物学的影响，将为深入了解松果体生物学提供有价值的信息。
Several studies have demonstrated that norepinephrine ( NE) is the critical neurotransmitter for the regulation of gene expression in the pineal gland. We studied the acute effect of NEs timulation in cultured rat pineal glands using Affymetrix rat genome microarray GeneChip probe arrays. Our data demonstrate that NE stimulation affects regulation of several genes; 44 and 29 genes were up- or down-regulated more than 2.5-fold, respectively. As shown in previous studies, arylalkylamine N-acetyltransferase, cyclic AMP responsive element modulator, jun-B and c-fos mRNA levels were increased by NE stimulation. Genes that were not previously reported and increased by NE stimulation in the pineal gland were protein tyrosine phosphatase, nuclear receptors, and activity and neurotransmitter-induced early genes. Unlike up- regulated genes, most of the down-regulated genes were not reported previously. Genes encoding enzymes involved in metabolism and structural proteins were decreased following NE stimulation. Identification of genes affected by NE stimulation would provide valuable information to understanding pineal biology fully.