Increased endothelial monocyte chemoattractant protein-1 and interleukin-8 in preeclampsia
Increased endothelial monocyte chemoattractant protein-1 and interleukin-8 in preeclampsia
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DOI：
10.1016/s0029-7844(02)02169-5
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发表时间：
2002-10-01
影响因子：
7.2
通讯作者：
Peng, T
中科院分区：
文献类型：
--
作者：
Kauma, S;Takacs, P;Peng, T
目的：为了检验先兆子痫与内皮细胞趋化因子单核细胞趋化蛋白-1和白细胞介素-8的产生增加有关的假设，单核细胞募集到血管内皮是必需的。从患有重度先兆子痫的妇女和足月的正常孕妇收集血浆，并测量单核细胞趋化蛋白-1，白细胞介素-8，和脂质过氧化物水平通过酶联免疫吸附测定和丙二醛测定。将人脐静脉内皮细胞与5%正常或先兆子痫患者的血浆一起培养，并测定培养基中单核细胞趋化蛋白-1和白细胞介素-8的产生。在重度先兆子痫的妇女中，单核细胞趋化蛋白-1、白细胞介素-8和脂质过氧化物的血浆水平升高（分别高1.5倍、2.5倍和4.5倍）。人脐静脉内皮细胞培养的血浆从先兆子痫的妇女显着增加单核细胞趋化蛋白-1（2.3倍）和白细胞介素-8（1.5倍）的生产相比，血浆从正常孕妇。抗氧化剂维生素E降低了子痫前期血浆处理的人脐静脉内皮细胞中单核细胞趋化蛋白-1和白细胞介素-8的产生，提示这些细胞因子的产生可能受到对氧化应激敏感的信号机制的调节。这些发现支持了先兆子痫妇女血浆中的循环因子激活内皮细胞单核细胞趋化蛋白的假设，1和白细胞介素-8的产生，虽然在这项研究中没有直接检查，但可能增加单核细胞粘附到血管内皮。(C)2002年由美国妇产科医师学会。
OBJECTIVE: To test the hypothesis that preeclampsia is associated with increased endothelial cell chemokine production of monocyte chemoattractant protein-1 and interleukin-8 necessary for monocyte recruitment to the vascular endothelium.METHODS: Plasma was collected from women with severe preeclampsia and normal pregnant women at term and measured for monocyte chemoattractant protein-1, interleukin-8, and lipid peroxide levels by enzyme-linked immunosorbent assays and malondialdehyde assays. Human umbilical vein endothelial cells were cultured with 5% plasma from normal or preeclamptic patients and the media assayed for monocyte chemoattractant protein-1 and interleukin-8 production.RESULTS: In women with severe preeclampsia, plasma levels of monocyte chemoattractant protein-1, interleukin-8, and lipid peroxides were elevated (1.5-fold, 2.5-fold, and 4.5-fold higher, respectively) compared with normal pregnant women. Human umbilical vein endothelial cells cultured with plasma from preeclamptic women significantly increased the production of both monocyte chemoattractant protein-1 (2.3-fold) and interleukin-8 (1.5-fold) compared with plasma from normal pregnant women. Monocyte chemoattractant protein-1 and interleukin-8 production was decreased by the antioxidant vitamin E in human umbilical vein endothelial cells treated with preeclamptic plasma, suggesting that the production of these cytokines may be regulated by signaling mechanisms sensitive to oxidative stress.CONCLUSION: These findings support the hypothesis that circulating factors in the plasma of women with preeclampsia activate endothelial cell monocyte chemoattractant protein-1 and interleukin-8 production, and although not directly examined in this study, may increase monocyte adherence to die vascular endothelium. (C) 2002 by The American College of Obstetricians and Gynecologists.