STIMULATORY EFFECTS OF PURIFIED MACROPHAGE COLONY-STIMULATING FACTOR ON MURINE RESIDENT PERITONEAL-MACROPHAGES
STIMULATORY EFFECTS OF PURIFIED MACROPHAGE COLONY-STIMULATING FACTOR ON MURINE RESIDENT PERITONEAL-MACROPHAGES
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DOI：
10.1016/0008-8749(86)90405-3
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发表时间：
1986-02-01
影响因子：
4.3
通讯作者：
SHADDUCK, RK
中科院分区：
文献类型：
--
作者：
AMPEL, NM;WING, EJ;SHADDUCK, RK
研究了一种不含干扰素和内毒素活性的巨噬细胞集落刺激因子(M-CSF)纯化制剂对常驻小鼠腹膜巨噬细胞的影响。M-CSF可诱导常驻巨噬细胞发生深刻的形态改变。这些变化包括细胞大小显著增大、膜皱缩和细胞质空泡化。此外，与1000U/mlM-CSF孵育72小时后，放射自显影显示巨噬细胞DNA合成显著增加(P<；0.001)，巨噬细胞单层蛋白含量显著增加(P<；0.001)。在对照巨噬细胞或暴露于重组干扰素-γ的巨噬细胞中，没有发现这些变化。(干扰素)。胞外酶5‘’-核苷酸酶(5‘’-NTD)和碱性磷酸二酯酶I(Apd)的低活性水平与巨噬细胞的某些功能，特别是肿瘤细胞毒性的表达有关。暴露于M-CSF的巨噬细胞单层显示，与对照组相比，5‘’NTD活性没有改变，ADP活性显著增加(P<；0.01)，并且51Cr释放试验没有显示出增强的杀伤肿瘤细胞的能力。另一方面，干扰素可显著降低巨噬细胞5‘’NTD(P<；0.05)和时程(P<；0.01)，并在巨噬细胞单层诱导显著的杀瘤活性。这些结果表明，纯化的M-CSF可诱导常驻巨噬细胞的功能活性和形态发生高度特异性的改变，这些改变与干扰素诱导的改变不同。
A purified preparation of macrophage colony-stimulating factor (M-CSF) free of interferon and endotoxin activity was studied for its effects on resident murine peritoneal macrophages. M-CSF was found to induce profound morphologic alterations in resident macrophages. These changes included a marked increase in cell size, membrane ruffling, and cytoplasmic vacuolization. Further, after 72 hr of incubation with 1000 U/ml of M-CSF, there were significant increases in macrophage DNA synthesis as measured by autoradiography (P < 0.001), and in macrophage monolayer protein content (P < 0.001). None of these changes was seen in control macrophages or those exposed to recombinant interferon-.gamma. (IFN). Low activity levels of the ectoenzymes 5''-nucleotidase (5''NTD) and alkaline phosphodiesterase I (APD) have been associated with certain macrophage functions, particularly the expression of tumor cytotoxicity. Macrophage monolayers exposed to M-CSF demonstrated an unaltered level of 5''NTD activity from controls and a significantly increased level of ADP activity (P < 0.01) and did not demonstrate an increased ability to kill tumor cells, as measured by the 51Cr-release assay. On the other hand, IFN caused significant decreases in both 5''NTD (P < 0.05) and APD (P < 0.01) and also induced marked tumoricidal activity in macrophage monolayers. These results indicate that purified M-CSF induces highly specific alterations in the functional activity and morphologic appearance of resident macrophages and these changes are distinct from those induced by IFN.