Metabolic process of di-n-butyl phthalate (DBP) by Enterobacter sp. DNB-S2, isolated from Mollisol region in China
Metabolic process of di-n-butyl phthalate (DBP) by Enterobacter sp. DNB-S2, isolated from Mollisol region in China
复制标题
肠杆菌属邻苯二甲酸二正丁酯（DBP）的代谢过程。
DOI：
10.1016/j.envpol.2019.113344
复制
发表时间：
2019
影响因子：
8.9
通讯作者：
Lilong Yan
中科院分区：
文献类型：
--
作者：
Ruixue Sun;Lei Wang;Yaqi Jiao;Ying Zhang;Xing Zhang;Pan Wu;Zhaobo Chen;Chengcheng Feng;Ying Li;Xiaoqian Li;Lilong Yan
邻苯二甲酸酯（PAEs）在环境中的累积已引起全球的关注。微生物降解是去除污染环境中邻苯二甲酸酯最有前途的方法。邻苯二甲酸二丁酯（DBP）是应用最广泛的邻苯二甲酸酯之一。本研究从东北黑土中分离到一株高效降解DBP的菌株Enterobacter sp. DNB-S2，在5 ℃和50 ℃条件下，7天内对500 mg L 1 DBP的降解率分别达到44.10%和91.08%。检测到一个新的中间体，苯甲酸正丁酯BP，这意味着一个新的降解途径。对菌株DNB-S2的全基因组进行了测序，以全面了解DBP的整个分解代谢过程。研究表明，DBP降解的关键基因包括酯酶基因、3，4-二羟基苯甲酸脱羧酶基因和邻苯二酚2，3-双加氧酶基因。中间体利用试验和实时定量聚合酶链反应（RT-qPCR）验证了拟定的DBP分解代谢途径。土著细菌DNB-S2是一个很有前途的种质资源，在低温下恢复PAE污染的黑土地区。本研究为PAE降解机制的研究提供了新的思路，并为PAE生物降解提供了丰富的基因资源。
The accumulation of phthalate acid esters (PAEs) in the environment has aroused a global concern. Microbial degradation is the most promising method for removing PAEs from polluted environment. Di-n-butyl phthalate (DBP) is one of the most widely used PAEs. In this study, a highly efficient DBP-degrading strain, Enterobacter sp. DNB-S2 was isolated from Mollisol in northeast China, and the degradation rate of 500 mg L1 DBP reached 44.10% at 5 C and 91.08% at 50 C within 7 days. A new intermediate, n-butyl benzoate BP, was detected, implying a new degradation pathway. The complete genome of the strain DNB-S2 was successfully sequenced to comprehensively understand of the entire DBP catabolic process. Key genes were proposed to be involved in DBP degradation, such as esterases, 3,4-dihydroxybenzoate decarboxylase and catechol 2,3-dioxygenase genes. Intermediate-utilization tests and real-time quantitative polymerase chain reaction (RT-qPCR) validated the proposed DBP catabolic pathway. The aboriginal bacterium DNB-S2 is a promising germplasm for restoring PAE-contaminated Mollisol regions at low temperature. This study provides novel insight into the catabolic mechanisms and abundant gene resources of PAE biodegradation.