Nuclear and cytoplasmic localizations of the adenomatous polyposis coli protein
Nuclear and cytoplasmic localizations of the adenomatous polyposis coli protein
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DOI：
10.1073/pnas.94.7.3034
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发表时间：
1997-04-01
影响因子：
11.1
通讯作者：
White, RL
中科院分区：
文献类型：
--
作者：
Neufeld, KL;White, RL
腺瘤性息肉病cc，li（APC）基因的突变是结肠癌发生的早期步骤，因为细胞内蛋白质的分布模式可以提供重要的功能线索，我们使用免疫荧光显微镜和生化分离的组合来确定AFC蛋白在上皮细胞中的位置，免疫荧光显微镜将全长APC蛋白置于细胞核和细胞质中。细胞核APC蛋白集中在离散的亚核区域，包括核仁，而细胞质APC蛋白集中在迁移细胞的前缘。APC蛋白与rRNA的共定位证实了核仁定位。这些免疫细胞化学结果已得到细胞分级的支持，这表明全长APC蛋白位于膜/细胞骨架和核组分中。
Mutation of the adenomatous polyposis cc,li (APC) gene is an early step in the initiation of colon cancer, Because the distribution pattern of a protein within the cell can provide important clues as to function, we have used a combination of immunofluorescence microscopy and biochemical fractionation to determine the location of AFC protein in epithelial cells, Immunofluorescence microscopy placed full-length APC protein in both the nucleus and the cytoplasm. The nuclear APC protein was concentrated in discrete subnuclear regions, including nucleoli, whereas the cytoplasmic APC protein concentrated at the leading edge of migrating cells. Colocalization of APC protein with rRNA confirmed a nucleolar localization, These immunocytochemical findings have been supported by cell fractionation, which demonstrated that full-length APC protein was located in both the membrane/cytoskeletal and the nuclear fractions.