Pyrroloquinoline quinone is a potent neuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity
Pyrroloquinoline quinone is a potent neuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity
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DOI：
10.1007/s11064-006-9257-x
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发表时间：
2007-03-01
影响因子：
4.4
通讯作者：
Adachi, Tetsuo
中科院分区：
文献类型：
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作者：
Hara, Hirokazu;Hiramatsu, Hideaki;Adachi, Tetsuo
吡咯喹啉醌(PQQ)是人体必需的营养物质，具有抗氧化剂的作用。活性氧物种(ROS)被认为是神经毒素6-羟基多巴胺(6-OHDA)引起的神经毒性的原因。在这项研究中，我们利用人神经母细胞瘤SH-SY5Y研究了PQQ对6-OHDA诱导的神经毒性的保护作用。当6-OHDA与PQQ共同作用于SH-SY5Y细胞时，PQQ可阻止6-OHDA诱导的细胞死亡和DNA断裂。使用ROS敏感的荧光探针二氢乙锭的流式细胞术分析表明，PQQ降低了6-OHDA诱导的细胞内ROS的升高。与PQQ相比，抗氧化维生素、抗坏血酸和α-生育酚没有保护作用。此外，我们还发现，与抗氧化剂维生素相比，PQQ能有效清除超氧化物。因此，我们的结果提示，PQQ对6-OHDA所致神经毒性的保护作用至少部分与其清除ROS，特别是超氧化物歧化作用有关。
Pyrroloquinoline quinone (PQQ), which is an essential nutrient, has been shown to act as an antioxidant. Reactive oxygen species (ROS) are thought to be responsible for neurotoxicity caused by the neurotoxin 6-hydroxydopamine (6-OHDA). In this study, we investigated the ability of PQQ to protect against 6-OHDA-induced neurotoxicity using human neuroblastoma SH-SY5Y. When SH-SY5Y cells were exposed to 6-OHDA in the presence of PQQ, PQQ prevented 6-OHDA-induced cell death and DNA fragmentation. Flow cytometry analysis using the ROS-sensitive fluorescence probe, dihydroethidium, revealed that PQQ reduced elevation of 6-OHDA-induced intracellular ROS. In contrast to PQQ, antioxidant vitamins, ascorbic acid and alpha-tocopherol, had no protective effect. Moreover, we showed that PQQ effectively scavenged superoxide, compared to the antioxidant vitamins. Therefore, our results suggest the protective effect of PQQ on 6-OHDA-induced neurotoxicity is involved, at least in part, in its function as a scavenger of ROS, especially superoxide.