PREFERENTIAL EXPRESSION OF VARIABLE REGION HEAVY-CHAIN GENE SEGMENTS BY PREDOMINANT 2,4-DINITROPHENYL-SPECIFIC BALB/C NEONATAL ANTIBODY CLONOTYPES
PREFERENTIAL EXPRESSION OF VARIABLE REGION HEAVY-CHAIN GENE SEGMENTS BY PREDOMINANT 2,4-DINITROPHENYL-SPECIFIC BALB/C NEONATAL ANTIBODY CLONOTYPES
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DOI：
10.1073/pnas.83.8.2589
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发表时间：
1986-04-01
影响因子：
11.1
通讯作者：
OGATA, RT
中科院分区：
文献类型：
--
作者：
RILEY, SC;CONNORS, SJ;OGATA, RT
新生小鼠的b细胞库包含主要的克隆型，这些克隆型在出生后的特定时间可重复表达。我们从三个2,4-二硝基苯特异性新生儿杂交瘤中分离并测序了重链和轻链cDNA克隆。其中两个杂交瘤（TF2-36和TF5-139）表达的独特型（Id）在出生后的第一天占主导地位，而第三个杂交瘤（TF2-76）表达的Id在出生后的第二周占主导地位。TF2-76杂交瘤蛋白的重(H)链可变(V)区是由7183 VH家族的一个成员编码的，7183 VH家族是Brodeur和Riblet [Brodeur， P. H. and Riblet， R. (1984) Eur]定义的8个小鼠VH基因家族之一。[j].中国生物医学工程学报，2014,32(2):922-930。该家族的成员在胎儿和新生儿肝脏前b细胞中重排的频率高得不成比例。由于7183 VH家族位于H链连接(J)区基因片段JH附近，其他工作者提出VH重排频率与与JH片段的距离有关。然而，由TF2-36和TF5-139杂杂瘤蛋白表达的两种较早出现的显性克隆型利用了36-60 VH家族的一个成员，可能是位于12号染色体上JH基因片段远端的VH 1210.7。由于20-30%的第3天二硝基苯特异性B细胞表达Id（TF2-36）或Id(TF5-139)，因此必须在发育早期高频使用VH 1210.7基因。这些结果表明，重排VH片段的利用受与JH距离以外的其他因素的强烈影响。
The B-cell repertoire in neonatal mice contains predominant clonotypes that are reproducibly expressed at particular times after birth. We have isolated and sequenced heavy and light chain cDNA clones from three 2,4-dinitrophenyl-specific neonatal hybridomas. Two of these hydribomas (TF2-36 and TF5-139) express idiotypes (Ids) that predominate during the first days after birth, and the third hybridoma (TF2-76) expresses an Id that predominates during the second week after birth. The heavy (H) chain variable (V) region of the TF2-76 hybridoma protein is encoded by a member of the 7183 VH family, one of eight families of murine VH genes that have been defined by Brodeur and Riblet [Brodeur, P. H. and Riblet, R. (1984) Eur. J. Immunol. 14, 922-930]. Members of this family have been found to undergo a disproportionately high frequency of rearrangement in fetal and neonatal liver pre-B-cells. Because the 7183 VH family is located close to the H chain joining (J) region gene segments, JH, other workers have proposed that VH rearrangement frequency is related to distance from JH segments. However, the two earlier-appearing predominant clonotypes expressed by TF2-36 and TF5-139 hybridoma proteins utilize a member of the 36-60 VH family, probably VH 1210.7, which is located distal to the JH gene segments on chromosome 12. Since 20-30% of day 3 dinitrophenyl-specific B cells express either the Id(TF2-36) or the Id(TF5-139), the VH 1210.7 gene must be utilized at high frequency early in development. These results indicate that the utilization of rearranged VH segments is strongly influenced by factors other than distance from JH.