Intestinal transportations of main chemical compositions of polygoni multiflori radix in caco-2 cell model.
Intestinal transportations of main chemical compositions of polygoni multiflori radix in caco-2 cell model.
复制标题
何首乌主要化学成分在Caco-2细胞模型中的肠道转运
DOI：
10.1155/2014/483641
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zhao R
中科院分区：
文献类型：
--
作者：
Yu J;Li N;Lin P;Li Y;Mao X;Bao G;Gu W;Zhao R
上下文。何首乌（Polygoni Multiflori）是由何首乌（Polygonum multiflorum Thunb）的根衍生而来。在东方国家使用了几个世纪。然而，对其主要成分的吸收研究却很少。目标。TSG、RL、PL和4种蒽醌类物质经上皮转运。材料与方法。Caco-2细胞单层是研究营养物质和外源生物肠道运输的一个成熟模型。结果。Caco-2细胞单层的表观通透系数（P app）为TSG (2.372 × 10−9)< EG (2.391 × 10−9)< EN (2.483 × 10−9)< PL (4.917 × 10−9)< RN (1.707 × 10−8)< RL (1.778 × 10−8)< AE （1.952 × 10−8）。因此，RN、RL和AE被认为部分吸收，而其他成分几乎没有被吸收。讨论与结论。糖苷的渗透性较苷元差。同时，TSG和EN的回收率较低，提示TSG和EN可能在Caco-2细胞中积累或代谢。计算机预测表明，吉布斯能（r = 0.751, p < 0.05）和成型热（r = 0.701, p < 0.05）与p app呈极显著正相关。
Context. Polygoni Multiflori Radix (PMR) is originated from the root of Polygonum multiflorum Thunb. and used in oriental countries for centuries. However, little researches pay close attention to the absorption of its major constituents. Objective. Transepithelial transport of TSG, RL, PL, and four anthraquinones is carried out. Materials and Methods. Caco-2 cell monolayer, which represented a well-established model for the study of intestinal transport of nutrients and xenobiotics, was used in this paper. Results. The apparent permeability coefficients (P app) in the Caco-2 cell monolayers were TSG (2.372 × 10−9) < EG (2.391 × 10−9) < EN (2.483 × 10−9) < PL (4.917 × 10−9) < RN (1.707 × 10−8) < RL (1.778 × 10−8) < AE (1.952 × 10−8). Thus, RN, RL, and AE were considered partly absorbed, while other constituents were hardly absorbed. Discussion and Conclusion. Glycosides showed poor permeabilities than aglycones. In the meantime, TSG and EN gave out poor recovery rates in this assay, which indicated that TSG and EN may accumulate or metabolise in the Caco-2 cells. In silico prediction indicated that Gibbs energy (r = 0.751, p < 0.05) and heat of form (r = 0.701, p < 0.05) were strongly positively correlated with P app.