A potential novel marker for human prostate cancer:: voltage-gated sodium channel expression in vivo
A potential novel marker for human prostate cancer:: voltage-gated sodium channel expression in vivo
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DOI：
10.1038/sj.pcan.4500796
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发表时间：
2005-09-01
影响因子：
4.8
通讯作者：
Djamgoz, MBA
中科院分区：
文献类型：
--
作者：
Diss, JKJ;Stewart, D;Djamgoz, MBA
电压门控钠通道α亚基（VGSC α s），特别是Na（v）1.7的功能性表达与前列腺癌（CaP）的强转移潜能相关。此外，VGSC在体外的活性直接增强了转移的过程。为了研究体内CaP中的VGSC α表达，对人前列腺活检组织（n> 20）进行免疫组织化学和实时PCR。VGSC α免疫染色在前列腺组织中是明显的，并且在CaP与非CaP患者中明显更强。重要的是，RT-PCR将Na（v）1.7鉴定为CaP中最显著上调（类似于20倍）的VGSC α，并且所得的受试者操作特征曲线证明了对该疾病的高诊断效力。可以得出结论，VGSC α表达增加显着在体内CaP和Na（V）1.7是一个潜在的功能诊断标志物。
Functional expression of voltage-gated sodium channel alpha-subunits (VGSC alpha s), specifically Na(v)1.7, is associated with strong metastatic potential in prostate cancer (CaP) in vitro. Furthermore, VGSC activity in vitro directly potentiates processes integral to metastasis. To investigate VGSC alpha expression in CaP in vivo, immunohistochemistry and real-time PCR were performed on human prostate biopsies (n>20). VGSC alpha immunostaining was evident in prostatic tissues and markedly stronger in CaP vs non-CaP patients. Importantly, RT-PCRs identified Na(v)1.7 as the VGSC alpha most strikingly upregulated (similar to 20-fold) in CaP, and the resultant receiver-operating characteristics curve demonstrated high diagnostic efficacy for the disease. It is concluded that VGSC alpha expression increases significantly in CaP in vivo and that Na(v)1.7 is a potential functional diagnostic marker.