Interleukin-6: a growth factor stimulating collagen synthesis in human tendon
Interleukin-6: a growth factor stimulating collagen synthesis in human tendon
复制标题
DOI：
10.1152/japplphysiol.00037.2010
复制
发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
Langberg, Henning
中科院分区：
文献类型：
--
作者：
Andersen, Mette Bisgaard;Pingel, Jessica;Langberg, Henning
人结缔组织，例如，肌腱，动态地响应于身体活动，在急性和长期运动或训练后胶原蛋白合成增加。胶原合成和降解的标志物以及几种潜在的生长因子的浓度已被证明在运动后人跟腱周围的腱周组织中显著增加。在这些潜在的生长因子中，白细胞介素-6（IL-6）显示出最大的倍数增加，这表明IL-6可能参与将机械负荷转化为人肌腱组织中的胶原合成。在本研究中，通过微透析法测定了14名男性志愿者跟腱周围组织中I型胶原转换标志物[I型前胶原NH 2-末端前肽（PINP）和I型胶原C-OOH末端端肽（ICTP）]的组织水平，这些志愿者将重组人IL-6（rhIL-6）注入一侧跟腱周围组织中，另一条腿作为对照。受试者被随机分配到休息组或运动组，在输注前进行1小时跑步机跑步（12 km/h，2%上坡）。除IL-6外，还测定了血清胶原周转标志物PINP、ICTP和I型胶原羧基末端肽（ICTX）的浓度。在运动组和休息组中，PINP的腱周浓度响应于rhIL-6输注而显著升高，表明IL-6的输注显著刺激人类腱周组织中的胶原蛋白合成。单纯的运动并不能增加胶原蛋白的合成。这表明IL-6参与胶原合成，并支持IL-6是健康人肌腱中结缔组织的重要生长因子的假设。
Human connective tissue, e.g., tendon, responds dynamically to physical activity, with collagen synthesis being increased after both acute and prolonged exercise or training. Markers of collagen synthesis and degradation as well as concentration of several potential growth factors have been shown to increase markedly in the peritendinous tissue around the human Achilles tendon following exercise. Of these potential growth factors interleukin-6 (IL-6) showed the largest fold increase, suggesting that IL-6 may be involved in transforming mechanical loading into collagen synthesis in human tendon tissue. In the present study the tissue levels of type I collagen turnover markers [procollagen type I NH2-terminal propeptide (PINP) and C-OOH terminal telopeptide of type I collagen (ICTP)] were measured by the use of microdialysis in peritendinous tissue of the Achilles tendon in 14 male volunteers, who had recombinant human IL-6 (rhIL-6) infused into the peritendinous tissue of the Achilles' tendon in one leg, with the other leg serving as control. Subjects were randomly assigned to either a resting group or an exercise group performing a 1-h treadmill run (12 km/h, 2% uphill) before infusion. In addition to IL-6, serum concentrations of collagen turnover markers PINP, ICTP, and COOH-terminal telopeptide of type I collagen (ICTX) were measured. The peritendinous concentration of PINP rose markedly in response to rhIL-6 infusion in both the exercise and the rest group, demonstrating that infusion of IL-6 significantly stimulates collagen synthesis in the peritendinous tissue in humans. Exercise alone did not result in an increased collagen synthesis. This indicates that IL-6 is involved in the collagen synthesis and supports the hypothesis that IL-6 is an important growth factor of the connective tissue in healthy human tendons.