Insulin decreases intracellular oxidative stress in patients with type 2 diabetes mellitus
Insulin decreases intracellular oxidative stress in patients with type 2 diabetes mellitus
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DOI：
10.1016/j.metabol.2006.01.003
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发表时间：
2006-05-01
影响因子：
9.8
通讯作者：
De Mattia, G
中科院分区：
文献类型：
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作者：
Bravi, MC;Armiento, A;De Mattia, G
受糖尿病影响的患者具有氧化应激和受损的谷胱甘肽（GSH）氧化还原状态。本研究的目的是确定胰岛素对2型糖尿病患者氧化应激的影响，氧化应激定义为细胞内GSH/GSH二硫化物（GSSG）比值降低和血浆硫代巴比妥酸反应物质（TBARS）引起的脂质过氧化。使用了两种实验干预措施：（1）测量10名2型糖尿病患者红细胞中胰岛素孵育后的GSH/GSSG比率，以及（2）测量14名2型糖尿病患者在体内高胰岛素血症期间的红细胞内GSH/GSSG比率和血浆TBARS从正常血糖高胰岛素钳夹研究中获得。我们证实我们的患者经历了氧化应激，如糖尿病患者与对照组相比细胞内GSH/GSSG比值的显著差异所示（13.56 +/- 3.84 vs 27.89 +/- 8.37，P <0.0001）。我们发现糖尿病患者的红细胞与胰岛素孵育2小时后，GSH/GSSG比值显著升高（11.56 +/- 1.98至15.61 +/- 2.62，P <0.001）。在钳夹研究中，GSH/GSSG比值在60分钟后已经增加，在120分钟后甚至更多（基线，15.04 +/- 4.19; 60分钟，19.74 +/- 6.33; 120分钟，25.33 +/- 11.15; P <0.0001）。相反，在血浆TBARS中未观察到显著变化（3.59 +/- 0.77至3.56 +/- 0.83，NS）。我们的结论是，2型糖尿病患者的胰岛素可以通过增加GSH/GSSG比值来降低细胞内的氧化应激。(c)2006年爱思唯尔公司All rights reserved.
Patients affected by diabetes mellitus have oxidative stress with an impaired glutathione (GSH) redox state. The objective of this study was to determine the influence of insulin on oxidative stress, defined as a reduced intracellular GSH/GSH disulfide (GSSG) ratio and lipid peroxidation by plasma thiobarbituric acid reactive substances (TBARSs) in patients with type 2 diabetes. Two experimental interventions were used: (1) measurement of GSH/GSSG ratio after insulin incubation in erythrocytes from 10 type 2 diabetic patients, and (2) measurement of intraerythrocytic GSH/GSSG ratio and plasma TBARS in 14 type 2 diabetic patients during an in vivo hyperinsulinemic condition obtained from a euglycemic hyperinsulinemic clamp study. We confirmed that our patients underwent oxidative stress as shown by the significant difference in intracellular GSH/GSSG ratio in diabetic patients as compared to controls (13.56 +/- 3.84 vs 27.89 +/- 8.37, P < .0001). We found a significant elevation in the GSH/GSSG ratio after 2 hours of incubation with insulin in erythrocytes from diabetic patients (11.56 +/- 1.98 to 15.61 +/- 2.62, P < .001). During the clamp studies, GSH/GSSG ratio had already increased after 60 minutes and even more after 120 minutes (baseline, 15.04 +/- 4.19; at 60 minutes, 19.74 +/- 6.33; at 120 minutes, 25.33 +/- 11.15; P < .0001). On the contrary, no significant changes were observed in plasma TBARS (3.59 +/- 0.77 to 3.56 +/- 0.83, NS). We conclude that insulin in patients with type 2 diabetes mellitus can reduce intracellular oxidative stress through increased GSH/GSSG ratio. (c) 2006 Elsevier Inc. All rights reserved.