Analysis of the cross-reactive anti-gp120 antibody population in human immunodeficiency virus-infected asymptomatic individuals.
Analysis of the cross-reactive anti-gp120 antibody population in human immunodeficiency virus-infected asymptomatic individuals.
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人类免疫缺陷病毒感染的无症状个体中交叉反应性抗 gp120 抗体群体的分析。
DOI：
10.1128/jvi.67.2.953-960.1993
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发表时间：
1993
影响因子：
5.4
通讯作者：
Kang,CY
中科院分区：
文献类型：
--
作者：
Hariharan,K;Nara,PL;Caralli,VM;Norton,FL;Haigwood,N;Kang,CY
本研究旨在分析两名人类免疫缺陷病毒（HIV）感染的无症状个体血清中交叉反应性抗gp 120抗体（Abs）的特异性和中和特性。针对通过使用gp 120 SF 2-和gp 120 IIIB-Sepharose柱连续亲和层析从HIV+血清中纯化的交叉反应性多克隆抗gp 120 Abs，建立了两组鼠单克隆抗独特型Abs（抗id MAb）。然后使用这些抗id单克隆抗体组从HIV+血清中亲和纯化独特型阳性（Id+）抗gp 120抗体。通过纯化回收这些Id+ Ab中的每一种表明，血清中存在多种独特的交叉反应性抗gp 120 Ab，浓度范围很广。免疫学和生物学研究表明，尽管所有的Id+ Ab都与gp 120 SF 2和gp 120 IIIB反应，但它们表现出独特的表位特异性和不同的中和活性。大多数Id+ Ab针对gp 120的CD 4附着位点（CD 4位点表位）中的表位，并表现出广泛的中和活性。另一方面，少数Id+ Ab对gp 120的V3区具有特异性，并表现出有限的交叉中和活性。总之，这些研究表明，CD 4位点表位特异性抗体在其克隆性、中和活性和血清浓度方面是异质的。这种异质性表明，针对CD 4附着位点的抗gp 120 Ab是响应于原始病毒分离株上存在的多个重叠表位和/或随时间出现的突变变体上的表位而开发的。
This study was undertaken to analyze the specificity and neutralizing properties of cross-reactive anti-gp120 antibodies (Abs) in the sera of two human immunodeficiency virus (HIV)-infected asymptomatic individuals. Two panels of murine monoclonal anti-idiotype Abs (anti-id MAbs) were established against cross-reactive polyclonal anti-gp120 Abs purified from HIV+ sera by sequential affinity chromatography using gp120SF2- and gp120IIIB-Sepharose columns. These panels of anti-id MAbs were then used to affinity purify idiotype-positive (Id+) anti-gp120 Abs from HIV+ sera. The recovery of each of these Id+ Abs by purification indicated that several idiotypically distinct cross-reactive anti-gp120 Abs are present in sera over a wide range of concentrations. Immunological and biological studies showed that although all of the Id+ Abs were reactive against gp120SF2 and gp120IIIB, they exhibited unique epitope specificities and distinct neutralizing activities. Most of the Id+ Abs were directed against epitopes in the CD4 attachment site (CD4 site epitopes) of gp120 and exhibited a spectrum of broadly neutralizing activities. On the other hand, a minor population of Id+ Abs showed specificity for the V3 region of gp120 and exhibited limited cross-neutralizing activities. Together, these studies indicate that the CD4 site epitope-specific Abs are heterogeneous with respect to their clonality, neutralizing activity, and concentration in sera. This heterogeneity suggests that anti-gp120 Abs to the CD4 attachment site are developed in response to multiple overlapping epitopes present on the original virus isolate and/or epitopes on mutated variants which emerged over time.