Nucleic acid binding proteins in highly purified Creutzfeldt-Jakob disease preparations.
Nucleic acid binding proteins in highly purified Creutzfeldt-Jakob disease preparations.
复制标题
高度纯化的克雅氏病制剂中的核酸结合蛋白。
DOI：
10.1073/pnas.90.12.5713
复制
发表时间：
1993
影响因子：
11.1
通讯作者：
Manuelidis,L
中科院分区：
文献类型：
--
作者：
Sklaviadis,T;Akowitz,A;Manuelidis,EE;Manuelidis,L
人类克雅氏病（CJD）是一种缓慢进展的痴呆症，引起这种疾病的传染因子的性质是有争议的。与痒病一样，没有鉴定出特异性的蛋白质或核酸。然而，指数复制和制剂菌株变异的生物学特征，以及物理大小和密度数据，与病毒结构最为一致。一种核酸-蛋白质复合物。通常认为核酸酶处理不会降低感染滴度，不会留下bbb50 bp的核酸。然而，从质量约为1.5 x 10(7)道尔顿的高纯度感染性复合体中可以提取500-6000 bp的核酸。因此，寻找可能保护病原体基因组的核酸结合蛋白是密切相关的。我们在这里使用西北印迹法显示，在高度纯化的感染性120S梯度组分中存在低水平的非组蛋白核酸结合蛋白。一些核酸结合蛋白在宿主中被明显编码，而其他的仅在克雅氏病中可见，而在未感染大脑的平行制剂中则没有。少量残留宿主Gp34（朊蛋白）不结合任何32p标记的核酸探针。大多数次要的“cjd特异性”蛋白具有酸性pI，这是许多病毒核心蛋白的特征。这些蛋白质值得进一步研究，因为它们可能有助于这些药物描述的独特抗性特性。这些蛋白质中是否有被病原体编码的还有待观察。
The nature of the infectious agent causing human Creutzfeldt-Jakob disease (CJD), a slowly progressive dementia, is controversial. As in scrapie, no agent-specific proteins or nucleic acids have been identified. However, biological features of exponential replication and agent strain variation, as well as physical size and density data, are most consistent with a viral structure--i.e., a nucleic acid-protein complex. It is often assumed that nuclease treatment, which does not reduce infectious titer, leaves no nucleic acids of > 50 bp. However, nucleic acids of 500-6000 bp can be extracted from highly purified infectious complexes with a mass of approximately 1.5 x 10(7) daltons. It was therefore germane to search for nucleic acid binding proteins that might protect an agent genome. We here use Northwestern blotting to show that there are low levels of nonhistone nucleic acid binding proteins in highly purified infectious 120S gradient fractions. Several nucleic acid binding proteins were clearly host encoded, whereas others were apparent only in CJD, but not in parallel preparations from uninfected brain. Small amounts of residual host Gp34 (prion protein) did not bind any 32P-labeled nucleic acid probes. Most of the minor "CJD-specific" proteins had an acidic pI, a characteristic of many viral core proteins. Such proteins deserve further study, as they probably contribute to unique properties of resistance described for these agents. It remains to be seen if any of these proteins are agent encoded.