Role of TNF-α, IL-1β and IL-6 in the local tissue damage induced by Bothrops asper snake venom:: an experimental assessment in mice
Role of TNF-α, IL-1β and IL-6 in the local tissue damage induced by Bothrops asper snake venom:: an experimental assessment in mice
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DOI：
10.1016/j.toxicon.2004.10.005
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发表时间：
2005-02-01
期刊：
影响因子：
2.8
通讯作者：
Gutiérrez, JM
中科院分区：
文献类型：
--
作者：
Chaves, F;Teixeira, CFP;Gutiérrez, JM
研究了细胞因子tnf - α、il -1 β和IL-6在小鼠Bothrops asper蛇毒急性局部病理作用中的作用。肌内注射这种毒液可引起肌肉中il -1 β和IL-6的增加。但未检测到tnf - α升高。己酮茶碱（PTX）是一种甲基黄嘌呤衍生物，可抑制tnf - α的合成，并用于评估这些细胞因子在毒素诱导效应中的作用。作为对照，PTX预处理可有效降低大肠杆菌脂多糖内毒素血症小鼠的致死率和血清中tnf - α的增加，但不影响内毒素模型中il -1 β和IL-6的增加。PTX不能降低血毒所致的致死率、出血、肌坏死、皮肤坏死和水肿。此外，抗细胞因子抗体预处理对减少毒液诱导的肌坏死和出血也无效。我们得出结论，tnf - α、il -1 β和IL-6在小白杨蛇毒诱导的小鼠急性局部病理效应的发病机制中没有显著作用，尽管这并不排除这些细胞因子在蛇毒诱导的局部病理的其他方面以及在组织损伤发生后发生的修复和再生反应中发挥作用的可能性。(C) 2004 Elsevier Ltd.版权所有。
The role of the cytokines TNF-alpha, IL-1beta and IL-6 in the acute local pathological effects induced by Bothrops asper snake venom was assessed in mice. Intramuscular injection of this venom induced increments in IL-1beta and IL-6 in muscle. but no elevations of TNF-alpha were detected. Pentoxifylline (PTX), a methylxanthine, derivative that inhibits the synthesis of TNF-alpha and antibodies against these three cytokines were used to assess the role of these cytokines in venom-induced effects. As a control, PTX pretreatment was effective at abrogating lethality and serum TNF-alpha increments in mice subjected to endotoxemia induced by injection of Escherichia coli lipopolysaccharide, although it did not affect the increment in IL-1beta and IL-6 in such endotoxic model. PTX failed to reduce lethality, hemorrhage, myonecrosis, dermonecrosis and edema induced by B. asper venom. Moreover, pretreatment with anti-cytokine antibodies was also ineffective at reducing venom-induced myonecrosis and hemorrhage. It is concluded that TNF-alpha, IL-1beta and IL-6 do not have a significant role in the pathogenesis of the acute local pathological effects induced by B. asper venom in mice, although this does not exclude the possibility that these cytokines play a role in other aspects of venom-induced local pathology, as well as in the reparative and regenerative responses that take place after the onset of tissue damage. (C) 2004 Elsevier Ltd. All rights reserved.