Protective Effects of Polyvinylpyrrolidone-Wrapped Fullerene Against Nitric Oxide/Peroxynitrite-Induced Cellular Injury in Human Skin Keratinocytes
Protective Effects of Polyvinylpyrrolidone-Wrapped Fullerene Against Nitric Oxide/Peroxynitrite-Induced Cellular Injury in Human Skin Keratinocytes
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聚乙烯吡咯烷酮包裹的富勒烯对一氧化氮/过氧亚硝酸盐诱导的人皮肤角质形成细胞损伤的保护作用
DOI：
10.1166/jnn.2021.19279
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发表时间：
2021
影响因子：
--
通讯作者：
Hyodo Sayuri
中科院分区：
文献类型：
--
作者：
Saitoh Yasukazu;Tanaka Asuka;Hyodo Sayuri
过量的紫外线(UV)暴露会加速皮肤炎症、黑色素生成、皱纹形成、光老化和通过氧化应激和脱氧核糖核酸损伤而致癌。这些对皮肤的有害影响与紫外线诱导的活性氧(ROS)和一氧化氮(NO·)产生的活性氮(RNS)密切相关。已知RNS可导致各种皮肤病，如红斑、黑色素生成、屏障功能降低和牛皮癣。这些皮肤病是主要的美容问题；RNS的控制，除了ROS的控制，对于保持健康的皮肤也很重要。在本研究中，我们研究了水溶性ROS清除剂聚乙烯吡咯烷酮包埋富勒烯(C60/PVP)对一氧化氮(NO·)和过氧亚硝酸盐(ONOO-)诱导的角质形成细胞损伤的保护作用。以NO·供体S-亚硝基-N-乙酰青霉胺(SNAP)为供体，观察C60/PVP对NO·/ONOO诱导的人皮肤角质形成细胞损伤和细胞内ONOO生成的保护作用。此外，我们还研究了C60/PVP对UVB诱导的细胞内ONOO-水平的抑制作用。C60/PVP对NO·诱导的细胞内ONOO生成增加和随后的细胞损伤具有抑制作用。此外，C60/PVP可显著降低UVB诱导的细胞内ONOO-水平的产生。这些发现表明，C60/PVP可以作为一种化妆品成分，用于预防由NO·/ONOO诱导的人皮肤角质形成细胞造成的皮肤损伤和/或功能障碍。
Excess ultraviolet (UV) exposure accelerates skin inflammation, melanogenesis, wrinkle formation, photoaging, and carcinogenesis through oxidative stress and deoxyribonucleic acid damage. These deleterious effects to skin are closely associated with UV-induced reactive oxygen species (ROS) and reactive nitrogen species (RNS) produced via nitric oxide (NO·) generation. RNS are known to be responsible for various skin disorders, such as erythema, melanin production, reduced barrier function, and psoriasis. These skin disorders are major cosmetic problems; RNS control, in addition to ROS control, is important for maintaining healthy skin. In the present study, we investigated the cytoprotective effects of polyvinylpyrrolidone-entrapped fullerene (C60/PVP), a water-soluble ROS scavenger, against nitric oxide (NO·) and peroxynitrite (ONOO-)-induced human keratinocyte injuries. Protective effects of C60/PVP on NO·/ONOO--induced cellular damage and intracellular ONOO- generation were evaluated using a NO· donor S-nitroso-N-acetylpenicillamine (SNAP) in human skin epidermal HaCaT keratinocytes. Furthermore, the suppressive effect of C60/PVP on UVB-induced generation of intracellular ONOO- levels was also investigated. C60/PVP exerted suppressive effects on intracellular increases in NO·-induced ONOO- generation and subsequent cellular damage. Additionally, C60/PVP significantly decreased the UVB-induced generation of intracellular ONOO- levels. These findings suggest that C60/PVP could be useful as a cosmetics ingredient for prevention of skin injuries and/or dysfunction from NO·/ONOO--induced effects in human skin keratinocytes.