Effects of 60-day bed rest with and without exercise on cellular and humoral immunological parameters
Effects of 60-day bed rest with and without exercise on cellular and humoral immunological parameters
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DOI：
10.1038/cmi.2014.106
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发表时间：
2015-07-01
影响因子：
24.1
通讯作者：
Buttgereit, Frank
中科院分区：
文献类型：
--
作者：
Hoff, Paula;Belavy, Daniel L.;Buttgereit, Frank
定期锻炼被认为对免疫功能有积极影响。相反，在太空飞行和模拟失重状态下（例如，卧床休息），免疫功能可能受到抑制。我们旨在评估模拟失重（第二次柏林卧床研究; BBR 2 -2）对免疫学参数的影响，并研究运动（有振动和无振动的抗阻运动）对这些变化的影响。24名身心健康的男性志愿者（20-45岁）在卧床休息60天内进行了抗振运动（n=7）、无振动抗振运动（n=8）和不运动（n=9）。在卧床休息前2天、卧床休息第19天和第60天采集血样。流式细胞术分析免疫细胞的组成。采用Luminex技术和ELISA/RIA分析血浆中细胞因子和神经内分泌参数。通过配对t检验确定随时间的一般变化，通过成对重复测量确定运动依赖性效应（方差分析（ANOVA））。合并所有受试者后，研究期间粒细胞、自然杀伤T细胞、造血干细胞和CD 45 RA和CD 25共表达T细胞数量增加，单核细胞数量显著减少;嗜酸性粒细胞活化趋化因子浓度显著降低。运动对淋巴细胞、B细胞尤其是B细胞IgD 1亚群以及IP 10、RANTES和DHEA S浓度的影响不同。我们的结论是，长时间卧床休息会显着影响免疫细胞群和细胞因子浓度。运动能够特异性地影响不同的免疫学参数。总之，我们的数据符合运动免疫保护的假设，并可能指向抗振运动的上级效果。
Exercise at regular intervals is assumed to have a positive effect on immune functions. Conversely, after spaceflight and under simulated weightlessness (e.g., bed rest), immune functions can be suppressed. We aimed to assess the effects of simulated weightlessness (Second Berlin BedRest Study; BBR2-2) on immunological parameters and to investigate the effect of exercise (resistive exercise with and without vibration) on these changes. Twenty-four physically and mentally healthy male volunteers (20-45 years) performed resistive vibration exercise (n=7), resistance exercise without vibration (n=8) or no exercise (n=9) within 60 days of bed rest. Blood samples were taken 2 days before bed rest, on days 19 and 60 of bed rest. Composition of immune cells was analyzed by flow cytometry. Cytokines and neuroendocrine parameters were analyzed by Luminex technology and ELISA/RIA in plasma. General changes over time were identified by paired t-test, and exercise-dependent effects by pairwise repeated measurements (analysis of variance (ANOVA)). With all subjects pooled, the number of granulocytes, natural killer T cells, hematopoietic stem cells and CD45RA and CD25 co-expressing T cells increased and the number of monocytes decreased significantly during the study; the concentration of eotaxin decreased significantly. Different impacts of exercise were seen for lymphocytes, B cells, especially the IgD 1 subpopulation of B cells and the concentrations of IP-10, RANTES and DHEA-S. We conclude that prolonged bed rest significantly impacts immune cell populations and cytokine concentrations. Exercise was able to specifically influence different immunological parameters. In summary, our data fit the hypothesis of immunoprotection by exercise and may point toward even superior effects by resistive vibration exercise.