Effects of an Environmentally Relevant Organochlorine Mixture and a Metabolized Extract of This Mixture on Porcine Sperm Parameters In Vitro
Effects of an Environmentally Relevant Organochlorine Mixture and a Metabolized Extract of This Mixture on Porcine Sperm Parameters In Vitro
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DOI：
10.2164/jandrol.108.006478
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发表时间：
2009-05-01
影响因子：
--
通讯作者：
Bailey, Janice L.
中科院分区：
文献类型：
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作者：
Campagna, Celine;Guillemette, Christine;Bailey, Janice L.
有机氯化学品存在于世界各地的环境中;然而，生活在远北地区的人口尤其面临风险，因为他们的传统饮食主要由受污染的动物（鱼，海豹，鲸鱼和北极熊）组成。有人认为，全球男性生育率正在下降，这可能是由于长期、低水平地接触环境污染物。本研究旨在使用1）15种有机氯的环境相关混合物和2）该混合物的代谢提取物的猪模型评估对新鲜精子生育力参数的影响。在第一个实验中，有机氯混合物（在10.5，14.7，和21微克/毫升多氯联苯[PCB]的相对浓度）减少精子的总活力，前向运动，和活力，并增加获能，自发顶体反应率，和胞浆钙水平，这表明该混合物改变了精子膜，是有害的精子功能。在第二个实验中，这种有机氯混合物的代谢提取物（在相对浓度为0.9，1.8，2.7，3.6，和4.5微克/升OH-PCBs）往往只减少精子总活力。在体外猪模型中，北极食物链中发现的有机氯混合物在高于环境水平的浓度下迅速对精子功能有害。相比之下，短期和生理相关的暴露于这种混合物的代谢提取物只引起有限的精子活力的不良影响。
Organochlorine chemicals are present in the environment worldwide; however, populations living in the Far North are particularly at risk because their traditional diets are mainly composed of contaminated animals (fish, seals, whales, and polar bears). It has been suggested that male fertility is globally declining, possibly because of chronic, low-level exposure to environmental contaminants. This study was designed to assess the effects on fresh sperm fertility parameters using the porcine model of 1) an environmentally relevant mixture of 15 organochlorines and 2) the metabolized extract of this mixture. In the first experiment, the organochlorine mixture (at relative concentrations of 10.5, 14.7, and 21 mu g/mL polychlorinated biphenyls [PCBs]) reduced sperm total motility, progressive motility, and viability, and increased capacitation, spontaneous acrosome reaction rates, and cytosolic calcium levels, suggesting that the mixture alters the sperm membrane and is detrimental to sperm function. In the second experiment, the metabolized extract of this organochlorine mixture (at relative concentrations of 0.9, 1.8, 2.7, 3.6, and 4.5 mu g/L OH-PCBs) tended to decrease only sperm total motility. In an in vitro porcine model, the mixture of organochlorines, as found in the Arctic food chain, was rapidly detrimental to sperm function at concentrations above environmental levels. In contrast, short and physiologically relevant exposure to the metabolized extract of this mixture induced only limited adverse effects on sperm motility.