Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in culture
Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in culture
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DOI：
10.1023/a:1008076306672
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发表时间：
1999-01-01
期刊：
影响因子：
2.2
通讯作者：
Yagasaki, K
中科院分区：
文献类型：
--
作者：
Zhang, GY;Miura, Y;Yagasaki, K
通过采用体外增殖和侵袭测定系统来检查茶和相关组分对癌细胞增殖和侵袭的影响。粉末状绿色、乌龙茶和红茶提取物剂量依赖性地抑制大鼠腹水型肝癌细胞系AH 109 A的增殖和侵袭，但不影响分离自大鼠的正常肠系膜衍生的间皮细胞（M-细胞）的增殖;较高浓度的粉末状乌龙茶和红茶提取物可以抑制另一种肿瘤细胞系L929的增殖。在10%小牛血清存在下，发现AH 109 A细胞穿透单层M细胞下方。当将在每种茶提取物口服插管后0.5、1、2、3和5小时获得的每种大鼠血清以10%的浓度添加到培养基中以代替小牛血清时，AH 109 A的侵袭和增殖均被显著抑制。这些离体结果表明茶有效成分在大鼠中的潜在生物利用度。此外，来自绿色茶的（-）-表没食子儿茶素没食子酸酯、（-）-表儿茶素没食子酸酯和（-）-表没食子儿茶素以及来自红茶的茶黄素和茶黄素没食子酸酯的混合物被证明是对抗AH 109 A侵袭和增殖的最有效的组分。这些结果表明，茶和相关组分对AH 109 A细胞的抑制作用是由于细胞特异性和细胞系对茶组分的更高敏感性。
The effects of teas and related components on the proliferation and invasion of cancer cells were examined by employing both in vitro proliferation and invasion assay systems. Powdered green, oolong and black tea extracts dose-dependently inhibited proliferation and invasion of a rat ascites hepatoma cell line of AH109A but did not affect the proliferation of the normal mesentery-derived mesothelial cells (M-cells) isolated from rats; higher concentrations of powdered oolong and black teas could restrain the proliferation of another tumor cell line of L929. The AH109A cells were found to penetrate underneath the monolayer of M-cells in the presence of 10% calf serum. When each rat serum obtained at 0.5, 1, 2, 3 and 5 h after oral intubation of each tea extract was added to the culture media instead of calf serum at a concentration of 10%, both the invasion and proliferation of AH109A were significantly suppressed. These ex vivo results suggest the potential bioavailability of effective tea components in rats. Furthermore, (-)-epigallocatechin gallate, (-)-epicatechin gallate and (-)-epigallocatechin from green tea as well as the mixture of theaflavin and theaflavin gallates from black tea were shown to be the most effective components against the invasion and proliferation of AH109A. These results show that the inhibitory effects of the teas and related components against AH109A cells are due to the cell-specific and higher sensitivity of the cell line to tea components.