Photoexcited cryptochromes interact with ADA2b and SMC5 to promote the repair of DNA double-strand breaks in Arabidopsis
Photoexcited cryptochromes interact with ADA2b and SMC5 to promote the repair of DNA double-strand breaks in Arabidopsis
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DOI：
10.1038/s41477-023-01461-6
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发表时间：
2023-07
期刊：
影响因子：
18
通讯作者：
Tongtong Guo;Minqing Liu;Li Chen;Yao Liu;Ling Li;Yupeng Li;Xiaoli Cao;Zhilei Mao;Wenxiu Wa
中科院分区：
文献类型：
--
作者：
Tongtong Guo;Minqing Liu;Li Chen;Yao Liu;Ling Li;Yupeng Li;Xiaoli Cao;Zhilei Mao;Wenxiu Wa
Cryptochromes (CRYs) act as blue-light photoreceptors that regulate development and circadian rhythms in plants and animals and as navigating magnetoreceptors in migratory birds. DNA double-strand breaks (DSBs) are the most serious type of DNA damage and threaten genome stability in all organisms. Although CRYs have been shown to respond to DNA damage, whether and how they participate in DSB repair is not well understood. Here we report thatArabidopsisCRYs promote the repair of DSBs through direct interactions with ADA2b and SMC5 in a blue-light-dependent manner to enhance their interaction. Mutations in CRYs and in ADA2b lead to similar enhanced DNA damage accumulation. In response to DNA damage, CRYs are localized at DSBs, and the recruitment of SMC5 to DSBs is dependent on CRYs. These results suggest that CRY-enhanced ADA2b–SMC5 interaction promotes ADA2b-mediated recruitment of SMC5 to DSBs, leading to DSB repair.
